Parentage And Genetic Diversity Studies In Bluefox (Alopex Lagopus) Using Microsatellite DNA Markers

Blue fox(Alopex lagopus)was for important for fur farming,creating lots of economy benefits every year in China.Blue fox farming had a long history in China,and the population was increasing each year.In order to further investigate the genetic diversity of Chinese blue fox farming,and improve the genetic management,this study screened total of 5 populations (n=288)using microsatellite markers to analyze statistically the DNA diversity of these colonies respectively.Allele frequency,heterozygosity value(H),polymorphism information content(PIC),Nei's standard distance was calculated.Dendrogram was constructed using the Neighbor-Joining methods.Father-son Parentage of 6 families in Greater Hinggan Mountains was identified.The results as following:1.By using fluorescent-labeling-based automated DNA sequencing and microsatellite polymorphism analyzing techniques,of 20 microsatellite loci,15 were proved polymorphic,reproducible and reliable in amplifying blue fox genomic DNA.They were credible genetic markers in blue fox genetic study.2.Five populations were observed 100 alleles in 15 microsatellite loci.Each microsatellite loci had 5～11 alleles,average number of allele was 6.67,allele frequency was 0～1.These results showed there were richer genetic diversity in blue fox farming.3.Among five populations Jinzhou's population had the richest genetic diversity, polymorphism information content(PIC),heterozygosity and effective allele were the highest.The following were Yichun,Greater Hinggan Mountains,Dlian and Harbin.But polymorphism information content(PIC),mean heterozygosity(H),effective number of alleles(Ne)did not have good consistency to affect genetic diversity.4.According to Nei's standard distance(1972),dendrogram was constructed using the Neighbor-Joining methods.Based on NJ dendrogram,5 Blue fox populations could be clustered into one group.The first group included Dalian and Greater Hinggan Mountains, the second clustered with Harbin,the third clustered with Jinzhou,finally clustered with Yichun.5.This study had identified Father-son Parentage of 6 families in Greater Hinggan Mountains by 15 microsatellite marks.The biological parents of 4 blue foxes without pedigree recording were found,and false recording of 2 individuals were modified.Cumulative chance of exclusion(CCE)and Paternity index were higher.In father-son set,fifteen markers will exclude the alleged parent with a probability of 0.9986.