Analysis On Factors Of Affecting Isoflavone Content Of Soybean

To survey the difference of isoflavones content in soybean seeds in different environment four cultivars were planted at five locations from 2005 to 2007.Two soybean cultivars were adopted in sand culture experiment,to analysis soybean seed isoflavone content changes under different N and P applied rate,and,to analysis isoflavone content sequential changes in soybean seed and leaf,meanwhile,to analysis molecular mechanism of isoflavone content change in the leaf.7 soybean cultivars were planted under different seeding rate and N-application rate in the Harbin area in 2007-2008 years,to analysis the agronomy measure influencing soybean isoflavone content.The results were as follows:1 Ecological difference of isoflavones content in soybean seeds among different genotypes1.1 The difference of daidzein content in different ecological conditionThere were significant difference in daidzein contents among years.There were significant difference in daidzein contents among planting sites.Daidzein contents were significantly positively correlated with latitude of the locations at the 0.01 probability levels. Different genotypes influence daidzein content at the 0.01 probability levels.Interaction of the factors influenced daidzein content greaty.Relative importance of factors affecting daidzein content were as follow:Year＞genotypex yearx location＞location＞year×location.1.2 The difference of glycitein content in different ecological conditionThere were significant difference in glycitein contents among years.There were significant difference in glycitein contents among planting locations.Glycitein contents were significantly positively correlated with latitude of the locations at the 0.01 probability levels. Different genotypes influence glycitein content at the 0.01 probability levels in every year of the three years.Interaction of the factors influenced glycitein content greaty.Relative importance of factors affecting glycitein content were as follow:Genotype×year×location＞year＞year×location＞genotype.1.3 The difference of genistein content in different ecological conditionThere were significant difference in genistein contents among years.Every year,there were significant difference in genistein contents among planting locations.Genistein contents were significantly positively correlated with latitude of the locations at the 0.01 probability levels.Different genotypes influence genistein content at the 0.01 probability levels in every year of the three years,at the 0.05 probability levels in three years.Interaction of the factors influenced genistein content greaty.Relative importance of factors affecting genistein content were as follow:Year×location＞location＞year＞genotype×year×location＞genotype.1.4 The difference of isoflavone content in different ecological conditionThere were significant difference in isoflavone contents among years.Every year,there were significant difference in isoflavone contents among planting locations at the 0.01 probability levels,at the 0.05 probability levels in three years.Isoflavone contents were significantly positively correlated with latitude of the locations at the 0.01 probability levels. Different genotypes influence isoflavone content at the 0.01 probability levels in every year and three years.Interaction of the factors influenced isoflavone content greaty.Relative importance of factors affecting isoflavone content were as follow:Year＞location＞year×location＞genotype×year×location＞genotype.2 Effect of planting measure on isoflavone contents in soybean seeds2.1 Effects of N and P on isoflavone contents in soybean seeds under pot culture experiment.Soybean isoflavone content under the low N condition was significant higher than the content under high N and CK.Isoflavone content changes under different P treatment existed cultivars difference.Isoflavone content were higher under low P processes in all two cultivars. But,these two cultivars exist difference in high P processes.In different years,there were different response rules of isoflavone content to N and P processes.2.2 Effect of planting measure on isoflavone contents in soybean seeds under field trialSeeding rate,N-application rate,genotype and their interaction influenced daidzein content significantly in 2007 and 2008.Seeding rate,genotype and all interaction influenced daidzein content significantly in union variance analysis.Through this experiment,we fond that Heinong37 cultivar,22 ten thousand plant/hm2 seeding rate,30 kg/hm2 N-application rate all could improve daidzein content.As the interaction existing,we should adopt special cultivation measure to different cultivars to improve daidzein content in soybean seeds.Seeding rate,N-application rate,genotype and their interaction influenced glycitein content significantly in 2007 and 2008.Seeding rate,genotype and all interaction influenced glycitein content significantly in union variance analysis.Through this experiment,we fond that Heinong 48 cultivar,27 ten thousand plant/hm2 seeding rate,30 kg/hm2 N-application rate all could improve glycitein content.As the interaction existing,we should adopt special cultivation measure to different cultivars to improve glycitein content in soybean seeds.Seeding rate,N-application rate,genotype and their interaction influenced genistein content significantly.Through this experiment,we fond that Dongnong 51 cultivar,22 ten thousand plant/hm2 seeding rate,30 kg/hm2 N-application rate all could improve genistein content.As the interaction existing,we should adopt special cultivation measure to different cultivars to improve genistein content in soybean seeds.Seeding rate,N-application rate,genotype and their interaction influenced isoflavone content significantly.Through this experiment,we fond that Dongnong 51 and Heinong 37 cultivar,22 ten thousand plant/hm2 seeding rate,30 kg/hm2 N-application rate all could improve isoflavone content.As the interaction existing,we should adopt special cultivation measure to different cultivars to improve isoflavone content in soybean seeds.3 Isoflavone accumulation in soybean and it's molecular mechanism analysis3.1 Isoflavone accumulation in soybean leafsSoybean isoflavone content reduced greatly after pouring nourishing cream.Soybean isoflavone content appeared a peak in Rl stage.3.2 There coordination fluctuation tendency.between alanin ammonialyase(PAL) gene relative expression quantity and isoflavone content in soybean leaf.3.3 Growth date number was significantly related to soybean seeds isoflavone content.