| This experiment are first introduced in mammalian molecular marker SRAP analysis approach to the Arctic Fox Daxinganling region keeping the genetic diversity of populations,using five pairs of primers SRAP marker analysis,the results are as follows:1.eml-mel analysis:LI between the GDL and the genetic similarity coefficient of the smallest, LYX groups AN and the genetic distance between the smallest,TS of the Net's genetic diversity index and Shannon's Information index maximum。em1-me1 analysis:Ht=0.3998;Hs=0.3116; Gst=0.2206;Nm*=1.7663。2.Em11-me6 use LGH and LYX analysis of genetic similarity between the smallest,GDL and QD groups,the smallest genetic distance between the genetic diversity index and Shannon's Information index of genetic information index LI＞LGH＞LYX,TS this group genetic diversity index and Shannon's Information index of genetic information is the highest index,em11-me6 the group analysis the genetic diversity of the total Ht=0.3770 of the genetic diversity within species Hs= 0.1624 coefficient of genetic differentiation among populations Gst=0.3158 of gene flow Nm *= 2.5790.3.Em11-me1 use QD and LYX analysis of genetic similarity between the minimum 0.0003,LI, and between groups LYX the smallest genetic distance 0.8883,genetic diversity index and Shannon's Information index index of genetic information are GDL＞LYX＞LI,hides genetic diversity index and Shannon's Information index index of genetic information is the most TS＞AN＞LYX.em11-me1 group analysis of the genetic diversity of the total Ht=0.2787 of the genetic diversity within species Hs=0.1637 coefficient of genetic differentiation among populations Gst= 0.2331 of gene flow Nm *=2.5534.4.Em2-me1 use TS and GDL analysis of genetic similarity between the minimum 0.0712,LI,and between groups LYX the smallest genetic distance,genetic diversity index and Shannon's Information index of genetic information index is the highest GDL,hides a variety of genetic traits index and Shannon's Information index index of genetic information is the highest TS.em2-me1 group analysis of the genetic diversity of the total Ht=0.4052 of the genetic diversity within species Hs=0.2455 coefficient of genetic differentiation Gst=0.3941,gene flow between groups Nm *=0.7689.5.Em1-me2 use TS and LYX analysis of the genetic similarity coefficient between the smallest, LGH and between groups LYX the smallest genetic distance,genetic diversity index and Shannon's Information index index of genetic information are LYX＞LYX,genetic diversity index and Shannon's Information index index of genetic information is the most LGH＞TS,em1-me2 analysis of the group's total genetic diversity within species Ht=0.3382 of the genetic diversity of Hs= 0.2571 coefficient of genetic differentiation among populations Gst=0.2398 of gene flow Nm *= 1.5847.6.Five pairs of primers for the groups together to carry out the results of the analysis of genetic informationWith five pairs of SRAP primers analysis of genetic diversity index of the combined application of SPSS 13.0 for the multiple comparison analysis for body weight traits,such as Nei's diversity coefficient of the GDL＞LGH＞LYX＞LI,GDL highest significantly large in LI(P=0.145) P＜0.2. Hides for traits such as Nei's diversity coefficient of TS＞QD＞LGH＞LYX＞AN,TS is the highest genetic diversity index,and significantly greater than AN(P=0.167) P＜0.2. In this study,Forestry TUQIANG Daxinganling Arctic Fox Fox Field Materials for the study groups,using sequencing analysis,using PCR-SSCP method to detect the Arctic fox OB,OBR, HCRT,HCRTR1,HCRTR2,CETP gene polymorphism in these five,the detection of genetic polymorphisms associated with growth traits of the analysis the following conclusions:1.Arctic Fox on the third of the ob gene exons were found on many loci,after the gene polymorphism and production traits in arctic fox multiple comparison analysis,OB gene is the impact of the Arctic fox's body weight,abdominal circumference,hides long,long Macrothelypteris major gene.2.Arctic Fox in the OBR gene exonⅣwas found on a multi-state sites,the gene polymorphism with growth traits of arctic fox correlation analysis showed that,OBR4 impact on the Arctic fox genes are the main carcass weight gene or major gene and its close linkage3.Arctic Fox in the seventh HCRTR2 gene exon was found on a multi-state sites,the gene polymorphism and production performance of the multiple comparisons show that the gene may be affected HCRT2 Arctic Fox body weight,abdominal circumference,hides another main efficiency of the gene or genes closely with the main chain.4.Fox in the Arctic HCRTR1 gene 5 'flanking sequence found on a multi-state sites,the gene polymorphism and production traits of the Arctic fox's correlation analysis showed that,HCRTR1 are possible effects of gene length and long Macrothelypteris major gene traits or close the main chain gene.5.In the CETP gene 5 'flanking sequence to detect the polymorphism,AA genotype,BB genotype, AB genotype,after multiple comparisons of their growth performance does not exist significant differences.6.Different sites of the combined results showed that the gene,OB gene OBR gene and AB(OB3) BB(OBR4) combined genotype has significant weight in favor of the selection traits;AA(OB3) AA(OBR4) the merger has a significant genotype traits conducive to the selection of the abdominal circumference;BB(ob3) BB(OBR4) combined genotype has significant long hair in favor of the individual needle selection traits;BB(ob3) BB(OBR4) combined genotype significantly longer hides in favor of individual traits the selection,OB and HCRTR1 gene gene interaction between the genotype in favor of the merger of the selection trait,OBR and HCRTR1 gene gene interaction between the combined genotype selection in favor of traits,these two genotypes combined selection the effect of a single genotype were higher than the effect of selection can be regarded as beneficial genes,but the combined effect of genotype selection OBR as OB gene and gene selection of the combined effect of genotype well.
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