| Canine parvovirus (Canine parvovirus, CPV) can cause acute hemorrhagic gastroenteritis of dogs and acute myocarditis of puppies. The antigen characteristic had been changing since CPV was isolated in 1978. The new mutant strain appeared continually and was prevalent in the world. More antigenic types of epidemic strains were isolated. The disease of CPV was one of the most serious acute infectious diseases which was harmful to the kennel business.On this account, it is really important for us to find a method which would detect the viruses rapidly and accurately ,as well as effective prevention.This paper settled the fundation of developing patent medicine against viral enteritis in dogs by the foundation of CPV,LAMP testing method and the research on the jatamans valeriana rhizome clinical treatment of viral enteritis in dogs. This paper presents that the LAMP detection method of CPV was attempted to establish for the clinical diagnosis of CPV as a new, fast and convenient means and provides the theoretical and experimental evidence for the clinical treatment of jatamans valeriana rhizome.This article consisted of three parts. In the first part, LAMP detection method was established. The detection speed was quicken and the detection specificity was improved. It provided a new method for clinical dianosis; in the second part, the antiviral mechanism, bacteriostasis in vitro of jatamans valeriana rhizome were studied to provide experimental evidence for the clinical treatment of viral enteritis in dogs; in the third part, the therapeutic efficacy of jatamans valeriana rhizome on dogs infected with CPV was studied to settle the foundation of develop patent medicine against viral enteritis in dogs.The main experiments and the results were as follows:1. The VP2 gene of CPV was downloaded from GenBank, the outer, inner and loop primers of LAMP were then designed in their conservative region, the genomic DNA of CPV was extracted serving as template for LAMP reaction. The result showed that the LAMP assay had a detection limit of 10–1 median tissue culture infective doses (TCID50)/ml. The primers used in this method were shown to be specific for CPV and did not amplify Porcine Parvovirus, Gosling Plague Virus and Canine Distemper Virus. The LAMP assay was more simple and convenient than PCR method, which was more useful technique for CPV detection in both laboratorial and pen-side detection.2. The analgesic effect of saruma henryi was studied through mice writhing test, mice models of hot-plate and toner test. The influence of saruma henryi on intestine peristalsis of mice was studied through-----. The effect of Valeriana jatamansi's drug serum on lymphocytes of mice was studied by MTT and the effect of drug serum on the metaboilic level and phagocytosis was studied by Neutral Red Phagocytosis. Saruma henryi possessed the roles of the activity of periph and center pain-killer, accelerating ntestine peristalsis, some immunologic enhancement and can strengthen the lymphocyte transformation, energy metabolism and phagocytosis.3. Dogs were vaccinated through oral administration of canine parvovirus(1ml/kg) and the CPV infecting model of dogs was established. The early stages, application jatamans valeriana rhizome powder 200mg/kg / day treated, 1-3d cured. Moderate symptoms group with rehydration salts, severe symptoms group with the infusion and gentamicin, 1-3d can be improved, and the cure rate and disease recovery better than the control group. It demonstrates that, the therapeutic effect of jatamans valeriana rhizome on curing CPV enteritis was exact and it can significantly improve the therapeutic effect of other drugs...
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