| Objective:this article Wenyanghuayukaiqiao intervention after MCAO/R in the rat, with autophagy inhibitor3-MA, autophagy revulsant rapamycin, specificity PKA inhibitors, observe different reperfusion time neural function defect scale in rats, cerebral infarction volume percent, the ischemia area, penumbra area, non ischemic area three parts autophagy level and autophagy protein Beclin1, the change of the BCL-2expression and p-after CREB expression changes, to explore this blood begin to understand party on ischemia-reperfusion injury in rats autophagy regulating protection effect.Methods:SD male rats using the method of improved line bolt (MCAO/R) module, randomly divided into8groups, divided into blank group, model group, explored the Wenyanghuayukaiqiao low dose group, explored in the Wenyanghuayukaiqiao dose groups, this Wenyanghuayukaiqiao high dose group, autophagy inhibitors, autophagy revulsant group, H-89. Observation of neurologic deficit score, cerebral infarction volume ratio, ischemic brain tissue pathologic morphology were analyzed. Autophagy is observed by transmission electron microscope at the same time; Using Western blotting testing different reperfusion time point, different areas of Beclin1, the BCL-2protein expression and the expression of p-after CREB protein; Using immunohistochemical qualitative observation Beclinl, BCL-2protein expression and p-after CREB protein expressionResults:the neural function defect scale, the intervention group at60min of ischemia and reperfusion0.5h,3h,6h,12h point in time, neural function defect score increased gradually. Traditional Chinese medicine (TCM) at6h reperfusion in Chinese traditional medicine group nerve impairment score lower compared with model group, has a significant difference (P<0.05). Pathological morphology found at6h reperfusion.cerebral edema of the peak. See autophagy body under the transmission electron microscope (AVI), autophagy-lysosome (AV2), Western blotting test Beclin1, the Bel-2expression in ischemia area, half dark area, the ischemia area0.5h reperfusion began to rise significantly, continue to increase in3h, the highest in6 h; Traditional Chinese medicine group and the group3-MA Beclin1content than the model group significantly reduced; P-after CREB protein expression of Chinese traditional medicine group IDV value than the blank group and model group increased (p<0.05). Immunohistochemical qualitative found that Chinese traditional medicine group of hippocampal CAl area Beclinl pyramidal cells all showed weakly positive (+) expression.Beclinl express the average optical density value of traditional Chinese medicine group were lower than that of model group. Chinese traditional medicine group compared with model group after CREB dyeing neurons of cerebral cortex and hippocampus CAl pyramidal cell P-after CREB all showed strong positive (+++) expression, expression intensity obviously stronger than the blank group and model group.Conclusion:this blood begin to understand can reduce ischemia reperfusion (MCAO/R) injury rats nerve function defect score, reduced cerebral infarction volume, at the same time reduce the cerebral infarction area and reducing brain edema, this blood can begin to understand the through cut Beclin1, the Bel-2expression and reduce the autophagy, this blood begin to understand that can be applied widely to the brain, the cerebral ischemia area, ischemia half dark area, the area decreased Beclin1, the Bel-2content at the same time, improve (MCAO/R) rat autophagy level, so as to reduce ischemic reperfusion. injury; This blood begin to understand in order to increase (MOCA/R) rat cAMP/PKA pathways of p-after CREB expression, widely effect, play a cerebral protective effect. |
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