Clinical Significance Of Low Density Lipoprotein Receptor 11 Protein In Carotid And Lower Extremity Arterial Stenosis Patients

Part I:The correlation between serum SorLA and carotid stenosisObjective:To verify the correlation between serum SorLA and carotid stenosis. Methods:Define patients with carotid artery stenosis as the experimental group, elderly people without carotid artery stenosis as control group. Western blot was used to detect the binding ability and specificity of selected antibody, ELISA was used to determin SorLA concentration of experimental group and the control group. Then evaluate the correlation between serum SorLA and carotid artery stenosis.Results:Western blot showed a single clear band and ELISA results concluded that the serum SorLA was 1.333+0.291ng/ml in patients and was 1.175+0.339ng/ml in control group, t-test revealed statistical significant difference (P= 0.029). Conclusion:Serum SorLA and carotid stenosis have some correlation.Part Ⅱ:The clinical value of the tissue SorLAObjective:To explore the correlation between serum SorLA and tissue SorLA, explore the correlation between serum SorLA and carotid plaque stability, and explore the correlation between tissue SorLA and carotid plaque stability.Methods:Carotid intima samples of patients accepted CEA were collected, SorLA was measured by ELISA method, plaque stability was judged by pathological biopsy.Results:There was no significant correlation between the concentration of tissue SorLA (0.317±0.108ng/mg) and serum SorLA (1.326±0.302ng/ml) concentrations (P=0.587). There was no significant correlation between carotid plaque stability and serum SorLA (P=0.694) and tissue SorLA (P=0.087).Conclusions:Serum SorLA and tissue SorLA were not significantly correlated, and carotid plaque stability was not significantly associated with serum and tissue SorLA.Part III:The value of serum SorLA in predicting intimal hyperplasia after CEAObjective:To explore the relationship between serum SorLA and intimal hyperplasia after CEA operation.Methods:A retrospective analysis of 80 patients accepted CEA were included. Their follow-up data and serum SorLA concentration were collected and calculated using t-test and ROC curve to determine the diagnostic value of serum SorLA.Results:Patients with severe intimal hyperplasia had a serum SorLA of 1.648+ 0.246ng/ml and patients without serious intimal hyperplasia had a serum SorLA of 1.278 +0.281ng/ml, t-test showed statically significance (P< 0.001). When chose 1.44ng/ml as the cut-off value of serum SorLA, the diagnostic method had a sensitivity of 90% and specificity of 73.5%.Conclusion:The preoperative serum SorLA was positively correlated with the postoperative intimal hyperplasia, SorLA concentration at 1.44ng/ml was the best index of intimal hyperplasia, its accuracy was acceptable.Part IV:the value of serum free SorLA in predicting restenosis after revascularization of lower limbsObjective:To explore the relationship between serum SorLA and postoperative restenosis in patients with lower limb ischemia.Methods:A prospective analysis was designed and all lower limb ischemia patients hospitalized between October 30th 2014 and January 29th 2015 were included. The patients were divided into high-risk group and low-risk group using the cut-off value at 1.44ng/ml. The follow up results about restenosis of two groups were collected in order to evaluate the application value of SorLA cut-off value.Results:There were 38 patients in low-risk group,1 had restenosis and 21 in high risk group,5 had restenosis, chi-square test showed statically significance (P=0.010). RR=9.0. Conclusion:Serum SorLA higher than 1.44ng/ml can predict the risk for restenosis after revascularization, it may become a warning indicator of restenosis after revascularization.