| Microfluidic chip integrates the selection, preparation, reaction, separation, detection and other basic operation of biological sample in chemical and biological fields into a several square centimeters (or even smaller) of microchip. It controls the flow by micro-channel network in order to complete the different chemical or biological reaction process, and analyzes the reaction products. It provides a new platform for the creation of new situation of biochemical analysis. In the structural design of the microfluidic chip, chip material selection as the primary problem for designers to consider, not only should consider in the process of material selection of chemical compatibility, corrosion resistance, light resistance, surface charged, insulation, molecular adsorption, thermal conductivity, stability, etc., but also to consider whether it has good manufacturability, whether for mass production of the chip. In the actual chip production process, can satisfy completely the requirements of the chip materials are hard to find, general designer will according to the requirement of the chip to use. With the research of microfluidic chips, cheap, easy to obtain materials is more and more get the favour of the designers. And the filter paper has the similar structure thread is a kind of ideal preparation of the base material at low-cost detection device. Thread materials has rich source, good biocompatibility and low detection background, simple post-processing, pollution-free characteristics, has been used in the production of microfluidic chip. The thread was tensile strength and better flexibility compared to the inorganic material and organic material, it don’t need to design pattern can fabrication the hydrophilic/hydrophobic channel, in addition, the thread itself with capillary effect, in without any external driving forces conditions to promote the sample solution transport in channel. In without external driving force, to ensure that the liquid can continuous flow within the channel, siphonage flow becomes the best choice, it can induce the continuous flow of the liquid in the channel. Therefore, based on the thread its own advantages and siphonage flow, this paper designs a new type of microfluidic analysis device, microfluidic siphonage flow and thread-based analytical device(S-μTAD). The device is combined with chemiluminescence, and the success realization inorganic ions, biological samples of quantitative analysis. The research work is divided into the following chapters:In the first chapter, the development of microfluidic technology, microfluidic chip material and production technology, the function unit of the microfluidic chip, microfluidic chip detection technology, the application of microfluidic chip were reviewed.In the second chapter, filter paper has the similar to structure of pure cotton thread, polyester cotton blend, polyester thread instead of filter paper fabrication microfluidic chips hydrophilic/hydrophobic channel, combined with PMMA support backing, microfluidic siphonage flow and thread-based analytical device was design. Fabrication of analysis devices is detailed introduction, measure and compared flow velocity and chemical luminescence signal of cotton thread, polyester cotton blend, polyester thread, ultimately determine the best microfluidic substrate materials.In the third chapter, on the basis of the second chapter, to determine the best materials fabrication S-μTAD and its feasibility was verified. Cr(III) catalyze luminol-H2O2 in alkaline aqueous solution produce chemiluminescence signal, based on polyester cotton blend fabrication S-μTAD, then luminol-H2O2 systems of chemiluminescence was studied by used this devices. This method was applied to the determination of chromium content in the capsule, satisfactory results are obtained, and S-μTAD feasibility was verified.In the fourth chapter, enzymatic reaction of glucose-glucose oxidase (GOD) generate H2O2 and chemiluminescence system of luminol-K3Fe(CN)6 coupling, and combining S-μTAD of the polyester cotton thread as the fabrication materials, determination of H2O2 produced by enzymatic reaction and indirect determination of the content of glucose, and successfully applied to the determination of glucose in human serum, satisfactory results were obtained.In the fifth chapter, cellulose was oxidized to oxycellulose with sodium periodate, generate 2,3-dialdehyde cellulose, enzyme protein amino and cellulose was oxidized to aldehyde covalent crosslinking reaction, implementation of immobilized enzyme. This experiment mainly use the hydroxyl on the cotton thread was oxidation with sodium periodate generate 2,3-dialdehyde, immobilization of glucose oxidase to the cotton thread by covalent cross-linking reaction, luminol-K3Fe(CN)6 system of chemiluminescence determine the H2O2 by enzymatic reaction, and indirect determination of the glucose content in human serum, compared with hospital data, the result is satisfactory.In the sixth chapter, on the basis of the fifth chapter, immobilization of uric acid oxidase to the cotton thread by covalent cross-linking reaction, luminol-K3Fe(CN)6 system of chemiluminescence determine the H2O2 by enzymatic reaction, and indirect determination of the uric acid content in human serum, compared with hospital data, the result is satisfactory.In the seventh chapter, conclusion.
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