| Constipation is a common gastrointestinal disease. The incidence of constipation increases with age. Constipation plagued the health of majority of elderly patients who with constipation. This leads to the quality of life of older people decreased. Constipation also makes the happiness of the elderly reduce. Constipation can be defined in many ways, and there is no uniform definition. Broadly speaking, dry stool, reduce the frequency of defecation, defecation difficulties, defecation laborious, defecation obstruction flu, and other abnormal bowel movements are being referred to as constipation. Constipation is not a disease, but a large group of symptoms collectively. Now, there are no consistent diagnostic criteria for constipation at home and abroad. There are many treatment methods for constipation. However, there is no fixed treatment. Constipation can lead to functional changes in the body, causing disease, thereby causing the aging process accelerate. Constipation prone to cause aging, it has been widely recognized at all times. However, its scientific relationship has not been confirmed. Therefore, it is of great significance to explore the relationship between constipation and aging.This paper studies the literature at home and abroad on the correlation of constipation and aging, and to understand the research progress of constipation and aging-related fields. We design to replicate aging animal model and constipation animal model through animal experiment. Then, both of the constipation indicators and aging indicators were detected. Through the above animal experiments, we intend to further explore the correlation between constipation and aging. Then, we through animal experiments to explore the effect of anti-aging for aging rats who with the Warming Yang Strengthen Spleen of Chinese medicine. We through animal experiments to explore the effect of laxative for constipation rats who with the Warming Yang Strengthen Spleen of Chinese medicine.Experimental research1. Experimental study of the correlation between aging and constipation.[Objective] We plan to detect the constipation indicators and aging indicators for both constipation rats and aging rats, in order to further analyze the correlation between constipation and aging. [Methods] Set up the laxative constipation rat and D-galactose rat model. We observed body weight of rats,24 hours stool weight and stool number, serum superoxide dismutase (SOD), measuring thymus index and spleen index, detecting hippocampus klotho protein levels use Western blot method, detecting c-Kit protein and SCF protein expression in colon tissues use Western blot method. We determined the intestinal transit function; calculate the percentage of activated charcoal suspension length and full-length intestinal propulsion. Then, the above rat model test results were compared with the normal group. [Results] There have significant differences (P<0.05)between the aging group and the normal group in general, body weight, serum superoxide dismutase (SOD), immune index and Western blot assay results.There have significant differences (P<0.05)between the laxative constipation group and the normal group in general, body weight,24 hours stool weight and stool number, measuring intestinal function, aspects of colon c-kit and SCF protein expression and so on.The constipation indicators and aging indicators test results have shown relatively consistent results between the laxative constipation model and D-galactose model group. [Conclusion] Both laxative constipation model and D-galactose model was successful model. Constipation and aging is relevant in rat’s model. Constipation may promote aging in rats, and aging rats mostly accompanied by some degree of constipation.2. The experimental research of anti-aging for aging rats that we take advantage of the Warming Yang Strengthen Spleen of Chinese medicine.[Objective] We plan to investigate the anti-aging effects for aging rats that we take advantage of Chinese medicine of Warming Yang Strengthen Spleen.[Methods] After aging model establishment end, then Warming Yang Strengthen Spleen particles will be divided into large, medium and small doses gavages. Western medicine group was given gavages mosapride, model control group were given equal doses of normal saline. After a month of continuous treatment, serum superoxide dismutase (SOD), thymus index and spleen index was measured. We’ll examine the hippocampus klotho protein expression levels by Western blot.[Results] Compared with the model control group, large, medium and small dose group SOD were significantly increased (P<0.05); thymus index of rats in high dose group, middle dose group and low-dose group were significantly increased (P<0.05); klotho protein in rats with high-dose group and medium dose group was significantly higher (P<0.05).[Conclusion] Chinese medicine of Warming Yang Strengthen Spleen has significant anti-aging effects, can significantly improve the detection of aging indicators of aging rat model.3. The experimental research of the treatment of laxative constipation with Warming Yang Strengthen Spleen method.[Objective] We want to investigate the therapeutic effects of traditional Chinese medicine Warming Yang Strengthen Spleen for laxative constipation rats.[Methods] After constipation model establishment end, then Warming Yang Strengthen Spleen particles will be divided into large, medium and small doses gavages. Western medicine group was given gavages mosapride, model control group were given equal doses of normal saline. After a month of continuous treatment, then, the 24 hours stool weight and stool number was measured. We will calculate the percentage of activated charcoal suspension length and full-length intestinal propulsion. And we’ll examine the colon tissue protein expression of c-Kit and SCF by Western blot.[Results] Compared with the control group, large, medium, small dose group and western medicine group 24 hours rat stool weight were significantly increased (P <0.01); 24 hours number of rat stool in high dose group, middle dose group, low-dose group and western medicine group were significantly increased (P<0.01); activated charcoal propulsion percentages in high dose group, middle dose group and low-dose group were significantly increased (P<0.05);c-kit protein in rats with high-dose group and middle dose group was significantly higher (P<0.05);SCF protein in rats with high dose group, middle dose group, low-dose group and western medicine group were relatively higher (P>0.05).[Conclusion] Chinese medicine of Warming Yang Strengthen Spleen has significantly therapeutic effects, can significantly improve the detection of constipation indicators of laxatives constipation rat model.Clinical Study[Objective] we want to investigate self Yiqi Bushen Recipe in treating senile constipation clinical efficacy and safety.[Methods] 80 cases of elderly patients with constipation were observed. All of them were undergoing treatment in our hospital anorectal clinic from March 2015 to January 2016. Wherein the treatment group,40 patients were treated with Yiqi Bushen Recipe, and the other 40 cases were treated with polyethylene glycol 4000 powder (Fusong).After four weeks of continuous treatment, the clinical efficacy of the two groups of patients, constipation clinical symptom scores and adverse reactions were observed.[Results] The total effective rate was 94.59% in the therapy group,69.23% in the control group, the difference was statistically significant (P<0.05); the therapy group constipation clinical symptom score (3.38±0.89) points, while in the control group (4.10±1.07) points, the difference was statistically significant (P<0.05); two groups were no serious adverse reactions.[Conclusion] The clinical efficacy was definite that self Yiqi Bushen Recipe in treating senile constipation of Spleen and kidney yang type.
|
PDF Full Text Request