The Role Of KLF17 In Hepatocellular Carcinoma And Its Effect On The Prognosis

Objective Liver cancer has become the world’s fifth most common malignant tumors, the incidence of liver cancer in China, has become the fifth in men, while ranked sixth in women; it’s mortality rate is second in men, while fifth in women. So the liver cancer in China is now one of the most malignant tumors second only to lung cancer and gastric cancer, annual incidence and deaths were approximately in 50 million and 40 million and continue to increase the trend, molecular biological markers of liver cancer research, for the prediction of the prognosis and new treatment targets of hepatocellular carcinoma (HCC). kruppel-like factors,KLFs,is a class having a zinc finger transcription factor of structure, the typical features of the structure is at the carboxyl terminus has three C2H2 zinc finger structure,widely involved in cell proliferation, apoptosis and differentiation of regulation. KLFs not only in the physiological process has an important role, recent studies found that it also plays an important role in the tumorigenesis and progression of the process, the new members of the KLF family KLF17 has been in a variety of tumors is proved for a tumor suppressor gene, downregulation of the expression and the tumor metastasis recurrence related. The expression of KLF 17 in gastric cancer, lung cancer and breast cancer tissue was lower than that in normal tissue, and the patients with high KLF17 expression in tumor tissue had longer survival time. In previous studies, it was found that KLF 17 could prevent the epithelial mesenchymal transition in HCC cell lines, thus influnce the invasion and migration of hepatocellular carcinoma cells. To further study KLF 17 in hepatocellular carcinoma, first study the KLF 17 function in the primary hepatoma carcinoma cells. Secondly study the relationship between expression and prognosis in patients with KLF 17 in hepatocellular carcinoma protein levels.Method and Result To study the mechanism of metastasis and recurrence of hepatocellular carcinoma by KLF17:the Hep11 and Hep12 cell lines were isolated from the primary tumor and the recurrence and metastasis of hepatocellular carcinoma patients. In the Transwell (Costa) of HCC cell invasion and migration assay. The results proved that the cultured from HCC metastasis ability of migration and invasion of tumor recurrence Hep 12 was significantly higher than that of Hepl 1. RNA and protein levels of Hep 12 in mRNA and protein levels were significantly lower than those in KLF 17. Expression of interference hep 11 KLF 17 quantitative PCR and Western blotting to detect interference effectively. Experimental results that KLF 17 interference group and unrelated sequence interference group compare migration and invasion were markedly increased. So it is proved that KLF17 has the function of regulating metastasis in liver cancer.Expression of the KLF 17 in hepatocellular carcinoma (HCC):paraffin specimen source in January 2009 to December 2011 in Peking Union Medical College Hospital of hepatobiliary surgery of hepatocellular carcinoma, diagnosed by disease science for hepatocellular carcinoma, all R0 resection, clear up the can for evaluation of patients with a total of 36 people. The expression of KLF17 in hepatocellular carcinoma was detected by the method of immune combination. Among 36 HCC patients,19 of them (52.8%) were 3-5,17 (47.2%) 0-2, KLF17 expression and gender, KPS score, Barcelona stage, differentiation, AFP and other clinical features. Cox regression analysis of gender, age, alpha fetoprotein, degree of differentiation, Barcelona stage, postoperative interventional chemotherapy and KLF17 expression of patients without progression disease free survival (DFS) and overall survival (overall survival, OS) effects of one of Barcelona points and KLF17 affect DFS and OS, Barcelona stage in DFS relative risk (RR) was 3.1, P< 0.05, KLF173～5 RR is 0.2, P< 0.05. Other factors no statistical difference, and in the OS, Barcelona stage RR was 5.6, P< 0.05, KLF17 RR is 0.1, P<0.05. Other factors without significant difference. KLF17 immunohistochemical score 0-2 points of DFS was 23.4+4.9 months (95% confidence interval for 13.2～32.3 months), DFS points of 3-5 was 39.3+4.2 months (95% confidence interval for 31.1～47.5 months), p<0.05. KLF17 score 0 to 2 points OS was 35.2+4.2 months (95% confidence interval 27.0-43.4 months) 3 to 5 OS was 46.9+3.2 (95% confidence interval 40.1-53.2 months). KLF173-5 points and 0-2 DFS and OS were significant sex difference (P<0.05). The above results confirmed that the expression of KLF 17 in the protein level and the prognosis of HCC were significantly correlatedConclusion KLF 17 has the function of regulating metastasis in liver cancer, its expression in protein level and the prognosis of liver cancer have a significant correlation.