| Although organ transplantation has become an effective treatment to save lifes and prolong the survival time of patients, the rejection after transplantation is the main cause which affects the final outcome. In order to suppress rejection and increase the survival rate of transplantation, expensive immunosuppressants have to be used in the remainder of patients' life. However, large amount of immunosuppressants can cause not only the side-effects, such as hyperpiesia and hepatorenal toxity, but also severe infection and malignant carcinoma ^specially in a long run. Nowadays the key methods to avoid or alleviate these side-effects are to induce immune tolerance or low immunoreaction state, and sustain the ability of immune response against other antigens (including anticancer, anti-infection and soon ). According to the theory of immune network, we designed the experiment to induce anti-idiotypic antibodies in recipient which has the same antigenicity as the graft antigens prior to transplantation, and observed the inhibitory effects of anti-idiotypic antibods on the rejection of mice graft.Aims:To induce the of anti-idiotypic antibodies in recipient which have the same antigenicity as the graft antigen prior to transplantation, and then to investigate their suppression effects on immune rejection in mice.Methods:1. Induction of isotypic heterostrain antibody(Abl) : BALB/c mice were immunized with C57BL/6 splenocytes to raise antibody(Abl) between the two difference strains.2. Induction of anti-idiotypic antibody (Ab2) : Immunoglobulin was purified grossly from polyclonal antisemm which had been identified with high titer, by saturated ammonium sulfate salting out. Then Immunoglobulin was further purified by DEAE-Sephadex A-50 column. In the experiment, Abl was cross-linked to KLH, Abl-KLH plus freund's adjuvant were used to induce anti-idiotypic antibody (Ab2) in BALB/c mice, and then Sandwich ELISA was used to detect Ab2.3. The BALB/c mice in which Ab2 had been induced were used as transplantation recipient,the survival time of transplanted graft > delayed-type hypersensitivity^ mixture lymphocytes culture and microlymphocytotoxicity were investigated to observe the effect of Ab2 induction on mice immunoreaction.Results:1. Determine the titer of Abl: By using indirect immuno fluorescent staining and complement dependent cytotoxicity test, the titer of Abl was > 1: 400.2. Determine the titer of Ab2: By using sandwich ELISA , the A^onm of the mice after immuned with Abl-KLH was 1.07 ?.1 3, over two times than that of the contol group which was 0.07 + 0.04.3. Influence of Ab2 induction on skin graft: The survival time of skin graft of Ab2 induction group is ( 26.5 ?1 .7 ) d, while ( 1 1 .2 ?2. 1 ) d in control group, ( 1 3.4 ?.6 ) d in CsA treated group, the difference was significant (P < 0.01).4. Influence of Ab2 induction on cardiac tissue transplantation: The survival time of transplantated cardiac tissue in Ab2 induction group is (31.5 ?.7) d, while ( 9.4 ?2.3 ) d in control group, ( 14.7 + 1.6) d in CsA treated group, the difference was significant (P < 0.01).5. Influence of Ab2 induction on delayed-type hypersensitivity: To compare the thickness of soles, the Ab2 induction group is ( 0.43 ?.17 )mm , while the control group was ( 1 .09 ?0.2 1 ) mm, the difference was significant6. Influence of Ab2 induction on mixed lymphocytes culture: Asvonm in Ab2 induction group was 0.24?.03,the control group was 0.35 + 0.04, the difference was significant (P < 0.01).7. Influence of Ab2 serum on microlymphocytotoxicity: the positive cell rate of the group containing Ab2 serum was 32. 5 ?. 5 %, the controlgroup was 86.7 ?.9%, the difference was significant (P < 0.01). Conclusions:1. Isotypic heterostrain antibody(Abl) can be successfully raised by immunizing BALB/c mice with C57BL/6 mice splenocytes.2. Abl-KLH with freund's adjuvant can induce anti-idiotypic antibody (Ab2) in BALB/c mice successfully.3. Compared with those in cont...
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