Acupuncture Effects On Acute Brain Injury And Neuronal Plasticity In Adult Rats

Object: To study the effect of acupuncture therapy on acute brain injury and to investigate the feasibility of the acupuncture in the early stage and its?influence on the never regeneration, reparation and neuronal plasticity following injury. Methods: Using fluid-percussion injury device, copy the model of the moderate brain injury of the rats. Select walking and balance tests to observe the behavioral obstacle from first day to 14th days after the injury dynamically~ meanwhile calculate the death rate; look into hematoma extend on the cerebral surface, HE stain and calculate the putrescence rate of the injured side cortex and hippocampus on the first day after the injury; measure the peak volume of the brain edema : Study the immunity reaction of bFGF protein by using ABC way of immunohistochemistry in the brain : Show SS mRNA and GAP-43 mRNA gene expressive variety by using in situ hybridization methods. and carry through statistic management by choosing the T test ,Chi-square test and Rank Sum test separately Result: After the brain injury, the rats have obvious active obstacle, acupuncture therapy can promote the resume of the movement function ; furthermore , the acupuncture will be better effect within lh than 3d after brain injury; Acupuncture can reduce secondary neuronal putrescence of the injured side cortex and the hippocampus; Acupuncture can increase immune reaction of bFGF in the brain, induce GAP-43 mRNA gene expression adjust upward, and it can affect abnormal expression of SS mRNA after the injury; However, acupuncture has no significant effect to the range of the brain hematoma, brain edema and death rate. Conclusion: This experiment discuss the acupuncture's influence to the acute brain injury from the general level to gene level separately, and combine the active function observation with morphology research, confirm the acupuncture's curative effect to the brain injury; Lay out that acupuncture can relieve the pathologic course after the brain injury, improve the regenerative environment of the injured neuronal, advance the functional mechanism of the reconstruction of the brain function.