The Injuries Of Multiple Organs In Thoracic Firearm Wound And The Mechanisms Of Them

The injuries of multiple organs in thoracic firearm wound and the mechanisms of themGUO Jianjun, LIU WeiyongThoracic firearm wounds happen commmonly in wartime and peacetime, the wounds are ususal serious and their mortilities are very high. This experiment try to explain the regulations of the changes through studying the changes of the pressures in carotis communis and jugularis externa, the plasma concentrations ofE, Nî–” TNF- a AIL? P ^ IL-6 and IL-8, and the pathological changes of tissuessuch as wounded lung lobe, adjoining lung lobe, opposing lung lobe, ventriculus sinister, liver, kidney, brain, pituitary gland, adrenal gland through HE, immunochemistry(ABC) and ISH in the rabbit's animal model with thoracic firearm wound. There are three parts in the experiment:Part one: Studying the changes of multiple organs in the thoracic firearm wound. 64 rabbits were divided into 8 groups averagely and at random, animals inevery group were all shot in their right-down chest through VI intercostals spacein order to make a kind of thoracic cavity wound with right-down lung lobe injuried. 8 groups were anatornied and sampled from tissues at the time when they were wounded O.Sh, 3h, 6h, 24h, 72h, 7d and 30d respectively. The pressures of carotis communis and jugularis externa were recorded, the concentrations ofE , NE, TNF- a ?IL?1 P ^ IL-6 and IL-8 were detected, the samples of tissues were examined through HE, immunochemistry(ABC) and ISH. The results show: the pressures of carotid and jugular ascended to 1.8+0.27 and 10 ?1.9 tunes at the monment of being shot, then descended rapidly to normal value and went ondescending until to the minimum, the pressures of some animals rose slowly and could reach the normal value after being shot 24h-72h, the pressures of some animals went down to die finally. The concentrations of E and NE in plasma both increased since 5min after being shot and reached their maxima twice times at 0.5h and 6h, there were no significant difference between them and their preinjuried values at the time of 7d after being shot. The concentrations of TNF-a , EL?1 3 , IL-6 and IL-8 in plasma reached their maxima at the time of O.Sh, O.Sh, 6h and 6h after being shot, and descended to normal at the time of 7d, 30d, 7d and 7d after being shot respectively. The expressions of TNF- a ?IL?1 3 >IL-6, IL-8 and their mRNAs could be examined at the time of O.Sh after beingshot, and reached maxima at the time of 6h after being shot, the concentrations begun descending at the time of 72h after being shot, and almost restored normal 7d after being shot. These expressions mainly lay in injuried lung lobe, ventriculus sinister, kidney and liver, there were also light expression hi some areas of brain, adrenal gland and pituitary gland. We can draw conclusions from these results that hi this kind of thoracic firearm wound, the concentrations inplasma of E and NE, TNF- a , IL?1 3 ^ IL-6 and IL-8 all increase obviously afterbeing shot and have their regulations in changes of the concentrations in plasma; The expressions in different tissues show that these factors have an important function in the multiple organs dysfunction syndrome hi the thoracic firearm wound.Part two: The functions of hemorrhage hi thoracic cavity and injury of lung in the thoracic firearm wound.40 rabbbits were divided into 5 groups averagely and at random: A: model group, B: artificial pneamothorax group, C: intercostal artery pre-ligated group, D: intercostal artery pre-ligated with artificial pneamothorax group, E: control group.Rabbits in group B were infused 50ml air into the right thoracic cavity before being wounded, rabbits in group C were ligated the 6th-9th intercostal arteries before being wounded, rabbits in group D were ligated the 6th-9th intercostal arteries and infused 50ml air into the right thoracic cavity before being wounded. The pressures of carotis communis and jugularis extema were recorded, theconcentrations of E % TNF- a in plasma were detected, the...