| The Study of Establishment of Small-for-Size Liver Transplantation Animal Models ,Mechanism of Grafts Injury and the Protective Effect of FK409 on Small-for-Size Grafts in the RatsDepartment of Hepatobiliary and Pancreatic Surgery and Organ Transplantation Centre, First Affiliated Hospital, Medical Faculty, Zhejiang University, China Ph.D Student: Liang Ting-Bo Supervisors: Zheng Shu-Sen ProfessorFan Sheung-Tat (Hongkong) ProfessorPart IThe Establishment of Small-for-Size Liver Transplantation Animal Model in the Rats and the Relationship Between the Graft Size and the Recipients Survival.Since the concept of liver transplantation appeared about forty years ago, many achievements have been attained in the fields of basic theory and clinical practice such as transplantation immunology and organ preservation etc. No one can forget that every progress was based on the experimental study. A successful animal model plays a key role in this concert. We can not image the state if no animal liver transplantation model have been developed. However, we faced an unmanageable problem now that the number of waiting-list recipients increases largely every year but the amount of donors is stable. In order to solve the above problem, a lot of manoeuvres were developed to expand the donor poor such as reduced-size liver transplantation, split liver transplantation, living donor liver transplantation and xenograft liver transplantation etc. Living donor liver transplantation, a successful method to treat the patients with end-stage liver disease, was only used for children in its early stage and then to be applied for the adult patients in 1991. Adult-to-adult living donor liver transplantation were widely used in the world at the present, but the grafts' volume always impedes the transplantation surgeons activities. Theliver grafts from donors with lower body weight than that of recipients always belong to small-for-size grafts. High morbidity and mortality occurred in the patients with small volume grafts after operation in living donor liver transplantation in clinic. The reasons of failure in small-for-size liver transplantation are due to the functional liver mass unable to load the recipients' metabolic demands. Besides the liver volume, which depends on the donors, the ischemia/reperfusion injury is definitely a reason to diminish the liver functional mass after graft implantation. To the whole grafts, this may be not important but in the small-for-size grafts it is a key factor. Unfortunately, the mechanism of grafts injury in small-for-size liver transplantation was not largely explored and documented. To establish a simple and stable animal model of small-for-size liver transplantation is the base to go on the above research.Materials and MethodsMale Sprague-Dawley rats, weighting from 160 to 380g, were chosen as donors and recipients. All the animals were given free to access to chow and water before operation. The experiments were conducted in accordance with the guidelines for the care and use of laboratory animals by the Hongkong Animal Committee.The experiment animals were divided into 6 groups: Group 1, 12 rats for liver anatomy observation; Group2, 6 rats of whole graft liver transplantation; Group 3, 8 rats of 50% ratio (graft weight to recipient's whole liver weight) liver transplantation for survival observation; Group 4, 27 rats of 30% ratio liver transplantation for survival observation; Groups 5, 16 rats of small-for-size liver transplantation using the technique of liver lobes resection on back-table to harvest small volume graft for the operation complications observation and Group 6, 78 rats of small-for-size liver transplantation using the technique of liver lobes resection in situ to obtain small grafts for operation complications observation. Pentobarbital were given intraperitoneally in the dose of 30-50mg/kg to anesthetize the animals. The whole graft liver transplantation models were performed according to Kamada's method. The small-for-size liver transp...
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