Enhancement Of Sensitivity Of Bladder Tumor Cells To MMC By Liposome Conjugated C-myc Antisense Oligonucleotides And Expression Of C-myc Oncoprotein In Bladder Cancer

The bladder tumor is frequently tumor in the urological series, the operation is common and the chemotherapy is assist in clinical treatment for bladder tumor. But a amount of studies showed 1hat chemotherapeutic agents induced mainly apoptosis for therapy in cancer cells, sensitivity of cancer cells far chenwdierapycbtided effectiveness of treating. At present howto enhance sensitivity of cancer cells for chemotherapy, decrease chemotherapeutic agent concentration and reduce ill effect are still a part of main investigations in our study. C-myc is the protooncogen, site in number 8 long ami of chromosome and has function for accelerating DNA duptocationaixl dose relationship with cells for proliferating. The high level proteins of c-myc have relative to some neoplasms occurring The expression level of c-myc gene is kept in order in normal cells, the expression of c-myc out of control is a important part for the cells cancerization. The antisense oligmudeotide is complementation DNA molecul with target gene mRNA and can suppress expression of target gene to inhibit breeding activity and induce apoptosis in tumor cdL\\fe study biology effect of liposome conjugated c-myc antisense oligonucleotides before treated MMC on Biu-S7cells and comparing with sense otigonudeotides. The inhibition of proliferation of Biu-87 cell was studied with cell growth curves and MIT method The oncogene products of c-myc expression was studies with SABC immunhischernicalK/ in Biu-87 cells treated by c-myc antisense oUgonudeotides. The apoptosis was studies with agarose gel DNA electrophoresis and flow cytametry in Biu-87cells treated by omyc andsense ohgonudeotdes and MMC Results showed that the Biu-87 cells were inhibiled appaienlly by c-myc antisense oligonucleotides with lipofectin compared with controls. The inhibitory rate was 632% completely at 48 hours (pO.Ol). After 12 hours the c-myc oligonucleotides could inhibit the protein expression of c-myc gene and displaied satisfactory introduction and blocking effects for omyc gene. The MTT method showed feat omyc antisense ohgonudeotides and MMC could inhibit Biu-87 cells to proliferate. After c-myc antisense oligonucleotides introducing, fee MMC treatting the Biu-87 cells resulted in a significant inhibition of the proliferation of cells and had difference effectively compared wife other controls (pO.Ol) The methods of agarose gel DMA electrophoresis and flow cytometry showed feat c-myc antisense oligonucleotides and MMC could induce apoptosis of cells, but the Biu-87 cells could be massive apoptosis by treated MMC at 8 hours after liposome conjugated antisense oligonucleotides at 24 hours.To study the biological behavior of c-myc gene in transitional bladder cancer patients, 50 surgical specimens of transitional bladder cancer were determined the expression of c-myc oncoprotein by SP immimohistochemk^tedTnique; among which 63 was 18, G: 16, G] 16. The follow -up clinical data were analysed to study relation about the c-myc oncoprotein egression wife patients prognosis and pathological grades. The results were showed feat c-myc oncoprotein expression of Gj,G2,Gi bladder cancer was 94.4%,75.0%,43.7% respectively (pO.Ol). The follow -up data wae showed that fee expression of omyc oncoprotein in bladder tumor is high among the deafe patients also.Conclusion; The liposome conjugated c-myc antisense oligonucleotides could suppress proliferation of Biu-87 cells and fee liposome is convenient for transferring antisense oligonucleotides and has high transfection efficiency. The c-myc antisense oligonucleotides could enhance sensitivity of Biu-87 cells fir MMC by liposome. Thec-myc antisense oligonudeoddes and MMC could induce apoptosis of Biu-87 cells The lower the pathological difflaentiation of the bladder cancer is, the higher the c-myc oncoprotein expression and the worse the clinical expedm of patient The expression of c-myc oncoprotein in bladder cancer has dose correlation with recurrence and pathydogjcal grades. The c-myc oncoprotein may be an excellent biologica...