The Relationship Between Helicobacter Pylori Or Telomerase And Cardiac Cancer, And Research On Molecular Mechanism Of Regulation

The two focuses that Helicobacter pylori (H.pylori) infection or telomerase activity being closely related with the occurrence of gastric cancer, H.pylori infection and telomerase activity had been studied widely and deeply in recent year. But the relationship between H.pylori infection or telomerase activity and the occurrence of cardiac cancer had not been reported yet. In order to investigate the relationship between H.pylori infection or telomerase activity and the development or occurrence of cardiac cancer, in this study we obtain 46 case of patients with cardiac cancer resection, compared with 16 cases of normal cardiac mucosa, 56 case of chronic cardiac inflammation, and 10 case of chronic cardiac inflammation with intestinal metaplasia (IM) in gastric biopsy. To make preliminary study in order to offer the theoretical basis to the research on molecular mechanism of cardiac cancer. This study was divided into two parts.First part: Relationship between the occurrence of cardiac cancerand H. pylori infection or telomerase activityMethods: 1. Improved Giemsa staining, HP-DNA-PCR and rapid biopsy urease test were used detect H.pylori infection in cardiac cancerous mucosa, tumor-adjacent mucosa, normal mucosa of stump, and the samples of gastric biopsy, H.pylori cytotoxin associated gene A( Cag A) was detected by PCR. 2. Telomerase activity, telomerase RNA( hTR)and telomerase reverse transcriptase (hTERT) were detected in cardiac cancerous tissue, tumor-adjacent mucosa % and normal mucosa of stump by the method of TRAPELISA, In situ hybridization and immunohistoche-mistry . Results:1 . The result of testing H.pylori infection by using three methods in each group indicates: the positive rate of H.pylori infection in 46 cases of cancerous tissue, tumor-adjacent tissue and 30 cases of mucosa of stump was 58.70% (27/46), 71.74% (33/46), 66.67%(20/30), there were no significant difference in the three groups (p>0.05), but was obviously higher than normal control groups of gastroscope 12.50%(2/16)(p<0.01).2 . The positive rate of CagA in 46 cases of cardiac cancer was 65.2 1 % (30/46), the positive rate of CagA of cardiac inflammation and cardiac inflammation with IM of gastroscope was 51.79% (29/56), 50.00% (5/10), the positive rate of CagA in normal mucosa of gastroscope was 6.66% (1/16), the positive rate each group mentioned above were significant difference compared with normol mucosa of gastroscope (p<0.05).3. In 33 cases of tumor-adjacent tissue in which H.pylori infection, 30 cases were CagA positive. 3 cases were negative. By statistical analysis, there was correlation between the H.pylori and the positive rate of CagA.(x2=33.98,p<0.01).4. The expression of rate telomerase activity in cardiac cancerous mucosa, tumor-adjacent mucosa and normal mucosa of stump was: 91.30% (42/46). 39.13% (18/46). 16.67% (5/30), mean of A value was 1.89 + 0.41, 1.49 + 0.43, 0.54?.45,respectively expression of telomerase activity. of cancer group compared with the group without cancer was significant difference (p<0.01).5. The expression rate of hTR and hTERT in cardiac cancerous tissue was obviously higher than that of without cancerous tissue (p<0.01), meanwhile there was correlation between the telomerease activity and expression of hTERT (#2=18.01 p<0.01). there was correlation between the expression of hTR and telomerase activity by the analysis of linear regression , >< =-0.230 ?.008* (F=48.39%,p<0.01)6. There was no correlation between H. pylori infection and the clinical or pathological index such as age of patients > sex. tumor size. tumor staging and depth of invasion (p<0.05).7. The average area value of positive cells of hTR in tumor-adjacent mucosa of negative for H.pylori was 0.08 ?0.06 , and that in rumor-adjacent mucosa of mild, moderate and severe H.pylori inflammation was: 0.20+0.10. 0.24 + 0.08 . 0.28 + 0.08. the expression of hTR in H.pylori positive mucosa was obviously higher than n...