| Purposes: To observe intakes of P-sodium-orthophosphate and P-chromium-orthophosphate at various concentrations by brain gliomas cell; To make thorough inquiry of the inhibition to the SHG44 cell by 32P-sodium-orthophosphate and 32P-chromium-orthophosphate at various concentrations; To find out the regularities about the accumulation and elimination in the major organs of rats after inter-brain injection with 32P-sodium-orthophosphate and 32P-chromic-orthophosphate; To investigate the therapeutic effects of intert-injection with 32P-chromium-orthophosphate on the neoplasia transplanted in the brain and hypoderma; To estimate the absorbed dose of the neoplasia body in rats after internal radiation; To study the pathological changes in the rat cells and tissues caused by the radiation exposure from 32P. In order to provide experimental evidence and calculating method of absorbed dose for the internal radiation therapy of gliomas.Method: Cultivated SHG44 cell at various concentrations (the ultimate concentrations of 32P-sodium orthophosphate culture medium were 0.006, 0.012, 0.018, 0.024, 0.036, 0.072, 0.089, 0.178, 0.356MBq/ml respectively for 48 hours , the ultimate concentrations of 32P- chromium-orthophosphate culture medium were 0.013, 0.026, 0.052, 0.104, 0.208, 0.416MBq/ml respectively), then cultivation was broken off and the cells were harvested (3H-TdR was added 12 hours before breaking off cultivation), Determination of specific activity of 32P and 3H was performed by liquid scintillation counting(The results were expressed in dpm, the same below); SHG44 cell was cultivated in ^P-sodium-orthophosphate and 32P-chromic-orthophosphate culture media with concentrations of 0.093MBq/ml and 0.104MBq/ml for different times(1.5, 3, 6, 12, 24, 48, 96hr), After suspending of contact with 32P, Cultivating was continued until 48 hours for the former 6 groups', the 96 hours' group 96 hours, The cells were harvested (3H-TdR was added 12 hours before breaking off cultivation), The determination of specific activity of 32P dpm and 3H dpm were carried out with homogenous liquid scintillation counting; SHG44 cellwas cultivate for 48 hr by 32P-sodium-orthophosphate or 32P-chromic-orthophosphate culture media respectively whose ultimate concentrations for the former were 0, 0.037, 0.093, 0.185, 0.37, 0.925, 1.85 MBq/ml and for the latter were 0, 0.052, 0.104, 0.208, 0.417, 0.834, 1.668MBq/ml, Afterwards, 2000 cells from the culture media were taken out and transplanted in the agar-culture-medium-plate and cultivated for 14 days, The numbers of cell colonies were counted; The solution of 50Ml(containing 3.145MBq 32P-sodium-orthophosphate) was injected into rats' brain of one group with a microsyringe, The solution of 50Ml(contammg 2.886 MBq 32P-chromium- orthophosphate) was injected into another group. Then the rats were killed at different time ranging from 20 minutes to 16 days, Their blood, heart, liver, spleen, lung, kidney, cerebrum and testis were sampled and made homogeneous samples which can be dosimetry by liquid scintillation counting, the specific activity of 32P dpm in each tissue was measured; The rats that were successfully transplanted with gliomas in the brain and hypoderma were given inter-glioma injection with 32P-chrornium-orthophosphate (for the rats with transplanted brain glioma were given 4.07MBq, for the rats with transplanted hypodermic tumors 18.5MBq). Their survival time and the changes of weight were observed; The absorbed dose of the brain and hypodermic neoplasia were calculated; The pathological sections and electron microscope sections of the tissues from both the therapy group and the controls group were made.Results: With the increase of the concentrations of both 32P-chromium- orthophosphate and 32P-sodium-orthophosphate in culture media the specific activity (dpm) of 32P in SHG44 cell elevated, at the same time the specific activity (dpm) of 3H decreased; with the lengthening of contact time with 32P-chromium-orthophosphate or 32P-sodium-orthophosphate, the specific activity (dp...
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