| Esophageal carcinoma is one of the most common tumors in the world. Every year, there are more then three hundred thousands patients with esophageal carcinoma occurred all over the world, in which Chinese people may have 50% population. According to the retrospective study of national tumor mortality from 1974 to 1976, the death rate was 13.05/100,000. But tumor motality was 1368.88/100,000 for kazak patients with esophageal carcinoma in Xinjiang, some of area was 90.75/100,000 highly. Kazak patients in Xinjiang is one of the highest incidence of esophageal carcinoma in nationality , and there were some characteristic of area and clan assembly. Esophageal carcinoma is now the most important cancer being prevented. We had finished a lot of work about its epidemiology and pathogenesis. Using cell biology and mocular biology techniques, founding esophageal carcinoma cell lines, we began as follows: some characteristic of cell lines (Eca9706), the antitumor effect of lycopene in vitro, and also determined oncogenes and tumor suppressor genes, chromosome loci specific loss of heterozygosity and microsatellite instability in esophageal carcinoma tissue.Part One: Biology of Esophageal Carcinoma1. Study on Primary Culture of Esophageal Carcinoma Cells in Kazak patientsObjectiveWe have tried to found cell lines of esophageal carcinoma of the Kazak patients by primary culture.Materials and MethodsFrom October 1999 to March 2001, 6 cases of fresh samples were collected from surgical resected of esophageal carcinoma in the firstteaching hospital, Xinjiang Medical University. There were four men and two women with the ages were 45 to 63 years old, Esophageal carcinomas without any treatment were diagnoed by surgry and pathology.The major agents were RPMI 1640, 15% serum of foetus bovins. Hank's liquid, lysis liquid. Primary culture: after operation in one hour, the tissues of esophageal carcinoma were put in the bottles and sent it to cell biology laboratory, then washed by hank's liquid. The tissiue piece were repaired by scissors and dipped in the hank's liquid about two hours, cut out small pieces by 2-3mm size. They were put into bottles, stored in 5%CO2, at 37℃. Purification of cells:After two days, there are a lot of cells around the tissue pieces including tumor cells and fibroblast cells, the old liquid was drawn, washed three times by hank's liquid, the lysis liquid was added at 37℃ in ten minutes. Then tumor cells kept their form, but the fibroblast cells become round. The old liquid was drawn, and hank's liquid was added to wash the tissue pieces lightly three times. The EGF was added and the cells were cultured. 6 cases of the Kazak patients with esophageal carcinoma cells from surgical resected samples were cultured and purified.ResultsThe primary cells 'of esophageal carcinoma and cultured conditions were found in vitro and get one case of primary cells of esophageal carcinoma successfully.ConclusionFresh tumor tissues and their pureness were the critical conditions of founding cells of esophageal carcinoma in vitro.Before culture, the tissiue pieces were wash about 5 times.It may be very important that concentration of foetus calf serum in culture liquid is 15%, fibroblast cells can not adapt to the 15% foetus calf10serum, but tumor cells adapted to it. The difference was used to purify cells.The time of lysis was about 5 minutes. After lysis, EOF were add into the culture liquid.It was difficult to found esophageal cancer cell lines, and we must go on researching the condition of culture.2. Study on characteristic of the esophageal cars' ma cell line and antitumor effect and mechanism of lycopene in vtii-o.-Objective1) To Study characteristic of a human esophageal carcinoma cell line (Eca9706).2) To research the antitumor effect and mechnism of lycopene in vitro.Materials and MethodsThe major agents were the esophageal carcinoma cell line (Eca9706), lycopene(69.6%), the other agents as the similar to the aboved paper.C...
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