| Malignant pleural mesothelioma (MPM) is a rare tumor, however, its morbidity is increasing in recent years. The cause of MPM has an intense correlation with contaction of asbestos. It is reported that 70 percent of MPM has had a history of asbestos contact. There is a thin layer of crocidolite in the surface soil somewhere in Da Yao County of Yunnan province. Because the natives don't know asbestos' hazard, clay containing asbestos was dug in order to make asbestos stoves, build roads, brush walls, combining the crust's destruction, much deforestation makes asbestos explosion .The residents contact directly or indirect asbestos in the daily life, so they would suffer from MPM after 20 to 40 years. Epidemiology found that mortality of the lung cancer and MPM in Dayao was increasingly higher than that of other regions and ten times more than that of foreign regions. The reviewing cohort investigation performing in the local in 1977-1983 and in 1987 to 1985 respectively showed that the mortality of pleural mesothelima was 8.5/105 and 17.75/105 a year, so Da Yao become an excellent, uncommon example which environment resulted in MPM.To date, it has been lack of systemically study about pathology, radiology and molecular biological occurrence and development of MPM owing to low morbidity, few clinical cases, particular suffering population and difficult to acquire cases and samples. MPM has been a serious disease affecting natives' health because its atypical presentation, difficult early diagnosis, higher malignance, rapid progress, higher mortality, poor prognosis. Thus it is imperative to make a MPM animal model in order to experimental study that could compensate many disadvantages in clinics. The final diagnosis of MPM must rely on pathology, which is the basis of radiology. Radiology is the optimal modality to detect MPM. CT, especially MSCT with better temporal resolution, spatial resolution, faster scanning, and thinner collimator can clearly delineate small lesions, being the most common non-invasive model to detect MPM.Part 1: Make of Induced Rats' MPM ModelObjective To study a convenient, practical MPM model which had a high inductionrate can been used for multiple experimental studies and is similar to human's MPM induced by crocidolite in Da Yao.Materials and methods Crocidolite was selected by winding several times, removing foreign matter to be grade one and made into a suspension with a concentration of 20mg/ml for experiment, which was similar to the distribution of the contamined air in Dayao. After sterilizing, 1 unit of penicillin was added before using it. After anesthetized with inhaling ether, 100 Wistar rats in the test group were injected asbestos fibrous mixed fluid into closed right chest cavity with 20mg/ml once per month, the overall volume of asbestos was 40 mg. 19 rats in the control group were injected into sterilized saline water of 1ml per time,totally 2ml.The animal's status was observed daily, weighted once a month, CT examination were performed when the rats were died or dying. After CT examination, the corpses of rats were dissected immediately and pathological changes were recorded. All animals was observed for 2 years. Apart from 2 rats died within first three months, 2 corpses eaten by other rats without dissection, radiology and pathological results and 4 rats living at present, 66 rats suffered from MPM among 92 experiment rats in the result. No MPM was observed in controls.Results There was significant for body weight change and body weight between male and female rats, there was no significance for body weight variation between the normal rats and the MPM rats. The overall induction rate was 71.74%. There was no significance between male rats and female rats for induction rate. The major causes about rat's death were pulmonary infection in control group and non-MPM group. The survival time of the first MPM rat was on 285th day; the most death period of MPM rats was in between 350th to 55oth days. The mortality rate in most death period was 80.3%. Th...
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