Studies On Immunoregulatory Activities And Adjuvanticity Of Sodium Houttuyfonate

The purpose of this research was to investigate the immunoregulation activities of sodium houttuyfonate. On the basis of searching the literatures about the immunoregulative activities of sodium houttuyfonate broadly, we first focused our researches on the functions and activation state of macrophages. After making some discoveries, we further investigated the signal transduction pathways of sodium houttuyfonate in macrophages, and some researches related with the adjuvanticity of sodium houttuyfonate.In order to reach our initial aim, the functions and activation state of macrophages were studied in the first section of this research. In the first section, the effect of sodium houttuyfonate on the level of lysozyme secreted by macrophages was first tested by bacteriolytic method; then the effect of sodium houttuyfonate on the level of tartaric acid resistant acid phosphatase produced in macrophages was investigated by analyzing the phenol generated in the reaction that was catalyzed by the enzyme; the third, the effect of sodium houttuyfonate on the phagocytic activity of macrophages was observed by microscopy; the forth, the effect of sodium houttuyfonate on the production of complement C4 from macrophages was tested by hemolytic method; the fifth, the effect of sodium houttuyfonate on the expression of C3b receptors on the surface of macrophages was analyzed by observing the formation percentage of zymosan wreath; the sixth, the effect of sodium houttuyfonate on the respiratory burst in macrophages was tested by flow cytometry; the seventh, the effect of sodium houttuyfonate on the production of IL-la and IL-lp from macrophages was tested by ELISA method.In the second section, the signal transduction pathways of sodium houttuyfonate in macrophages were investigated. First, the changes of the concentration of free calcium ion in macrophages caused by sodium houttuyfonate was analyzed by spectrofluorophotometry; second, the effects of sodium houttuyfonate on the phosphoralation of CaMK II, CREB and ERK 1/2, and the expression of Fos were analyzed by Western-blotting method.It was discovered in the first section that the production of IL-1 was promoted by sodium houttuyfonate treatment. As we know, there are two kinds of cytokines that are most important for the activation and proliferation of T cells, one is the IL-1 generated by macrophages which is the co-stimulator for the activation of T cells, the other is the IL-2 produced by activated T cells themselves, hi the third section, in order to further investigate the immnoregulation activities of sodium houttuyfonate, the action of sodium houttuyfonate on the proliferation of cells in spleen was tested by MTT spectrophotometry;second, the activity of sodium houttuyfonate on the production of IL-2 from the cells in spleen was investigated by measuring the proliferation of thymocytes promoted by IL-2 secreted by the cells in spleen; the third, the action of sodium houttuyfonate on the production of IL-2 from the purified T cells in peripheral blood was measured by ELISA method.The researches in the forth section dealt with the adjuvanticity of sodium houttuyfonate. First, the effects of consecutive medication of sodium houttuyfonate on the generation of anti-sheep serum blood cell antibodies IgG and IgM were tested by hemolytic method; second, the typical adjuvanticity of sodium houttuyfonate was analyzed by ELISA method.It was found in the first section that the production of lysozyme, tartaric acid resistant acid phosphatase, complement C4 and IL-10, the phagocytic function of macrophages, and the respiratory burst in macrophages were enhanced or promoted by sodium houttuyfonate, however, the expression of C3b receptors on the surface of macrophages and the production of IL-1a from macrophages were not significantly affected. The increase of the production of lysozyme, acid phosphatase and IL-1, and the respiratory burst are all the signs of the activation of macrophages. The respiratory burst may be caused by phagocytosis, however, the ph...