| IntroductionNerve tissue is the most susceptible tissue to ischemia in body. Cerebral ischemia is very common because of the low cerebral perfusion caused by myocar-dial infarction, shock, apnea and some surgery operations. Irreversible injuries often happened after some time of cerebral ischemia even if the perfusion has recovered , and there are the reperfusion injuries after the perfusion recovery. Hippocampus and some ischemia - sensitive areas are easy to happen the delayed neuronal death ( DND) ; the cusp period is among the third day and the seventh day after reperfusion.CNS injury from ischemia and reperfusion is proposed to occur via multiple interrelated mechanisms including excessive extracellular accumulation of the ex-citotoxin glutamate, an increase in intracellular Ca2+, and oxidative stress. These processes contribute to the generation of reactive oxygen species that damage protein, lipid, and DNA. Depending on the severity of the cellular damage; the cell may activate repair or protective mechanisms or undergo cell death by necrosis or apoptosis.The study on the protection of injuried nerve cells induced by ischemia/ reperfusion has been one of the hot spots of medical research at present. Apart from the traditional pharmacotherapy and Cryotherapy, now ischemic force - adaptation is the mettod undergone more research, that 4s, to give ari adaptation ischemic pretreatment before the occurrence of cerebral ischemia which is foreseeable , but it is unpracticed in clinical practice. And the application of chemo ?pretreatment is limited because of the toxicity of pretreatment materials.The stellate ganglion block ( SGB) which can modulate the functions of nerve,'endocrine and immune' system has been extensively applied on the pain 'and other disease recently with satisfactory effect, but its prevention and cure effect on cerebral ischemia reperfusion injury ( CI/RI) which is unreported in the world is still a new direction to study. Therefore, we conducted the series studies to discuss the prevention and cure roles on the rat with CI/RI and its mechanisms by imitating SGB in human being with the transection of cervical sympathetic trunk (TCST).These studies were to provide a theoretical and experimental basement on further explore the prevention and cure of CI/RI in clinical practice.Methods1. Experimental animal ;60 Wistar rats with body weight 200g - 300g were provided by the experimental animal center of China Medical University.2. Main Reagent and Apparatus;Pentobarbital sodium; Hydrochloric Bupivacaine; The 1st Ab,2nd Ab SP staining kit and DAB dying liquor used in MAP2, NF - KB immunohistochemi-cology; ET, CGRP, IL - 8 radio - immunity analytical assay kit; Primers of ICAM - 1 internal reference (3 - actin; Kit of ICAM - 1 and other reagent in RT -PCR. A -200 Ds Electric balance; Dupont ST -21 hypothermia high speed centrifuger; PTC - 100 PCR amplification device; Kodak ID gel imaging analytical system; GIS -700D digital gel scouding analytic system; DYY - III31A e-lectrophoresis apparatus; S500P ultraviolet, the visible continuous spectro-graph; RM - 6000 physiological recorder; NiHON electromagnetism blood stream meter; Olympus light microscope; JUNG - CM 1800 constant fridge microtome; OPTON - EM10C transmission electron microscope; LKB - V ultra microtome; HEIDOLPH DIAX900 homogenate apparatus; SN682 radio -immunityyauto -recorder; Olympus AX 70 microphotograph system; MetaMor-ph/Olympus DP10/BX50 micro image analytical system; B - Brown tracepump; anatomic microscope; animal operation instrument.3. Experimental protocols;Rats were randomly divided into three groups: 1) control group ( A, n = 4), 2) CI/RI group (B, n=28), 3) TCST group (C, n=28). Group B and C then divided equally into 7 subsections separately according to the time of reperfusion. Group A undergone operative procedure only, not clamped, not scorched or not transected. The CI/RI models were made in Group B and C according to the method of four - vessel occlusion ( common car...
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