Study On The Relation Of Dengue 2 Virus' Receptor To Mesenteron Infection Barrier In Aedes Albopictus And Culex Pipiens Quinquefasciatus

Dengue fever/dengue hemorrhagic fever is one of the most important arbovims diseases in the world. In China, Aedes albopictus is major vector of dengue fever in Chinese mainland; Culex pipiens quinquefasciatus, which distributes wildly in southern China, can't infect with dengue virus. Study on the dengue virus infection difference between Ae. albopictus and Cx. pipiens quinquefasciatus shows that such difference is due to mesenteron infection barrier; furthermore, the virus receptor on the mosquito's midgut is presumed as the primary factor which responses to mesenteron infection barrier. Researches of the virus receptor on the mosquito's midgut will help us understanding the mechanism of mesenteronal infection barrier and the dengue fever infection cycle through mosquito's vector.In this paper, Ae. albopictus and Cx. pipiens quinquefasciatus are taken as the study objects. In order to study the contribution of virus receptor on the mosquitoes' midguts to mesenteronal infection barrier, the binding character of dissection midguts and BBMF (Brush Border Membrane Fraction) from mosquitoes with dengue type 2 virus had been tested through binding experiments; VOPBA is also adopted to identify the dengue type 2 virus' receptor on mosquitoes' midgut. The results are as followed: binding character of dengue type 2 virus with dissection midguts from these two kinds of mosquitoes displays same characters, but binding character of dengue type 2 virus with BBMF from these two kinds of mosquitoes displays distinct characters; moreover, one protein, approximately to 35kDa, could be identified to be the dengue type 2 virusreceptor on the midguts of Ae. albopictus, but no receptor could be identified on the midguts of Cx. pipiens quinquefasciatus. The results hints us that virus receptor should be primary factor which responses to mesenteronal infection barrier and binding character of mosquitoes' BBMF with virus. VOPBA of four serotypes dengue virus on the C6/36 cells and immunoprecipitation of dengue type 2 virus with protein from C6/36 cells are also studied. On the C6/36 cells, one non-Glyprotein protein approximately to 35kDa could be identified as receptor of dengue virus; and no receptor diversity could be found among four serotype dengue virus.