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Experiment Study Of Human Hair Keratin Promoting Injury Spinal Cord Restore In Adult Rat

Posted on:2004-05-03Degree:DoctorType:Dissertation
Country:ChinaCandidate:X J XuFull Text:PDF
GTID:1104360092499114Subject:Human Anatomy and Embryology
Abstract/Summary:PDF Full Text Request
The formation of necrosis cavum and glial scar after spinal cord injured was one of important factor that affected regeneration of nerve fiber and neuron. If want to changed the stat, must be operatived decrease secondary injury, limited proliferation rate of neuroglial cell, promoted neuron regeneration,after spinal cord injury. So the study used human hair keratin (HHK) as a tissue-engineering spinal cord bridge-linking substance and implanted it to area of acute spinal cord injury (SCI) in adult rat in order to built an experiment model. Using techniques of light-microscopy histology processing, transmission electro microscopy, cytochemistry, suppression subtractive hybridization (SSH), and other way to compare the tissues in group 1 serving as normal control group, group! serving as injured group, groupS serving as injured and implanted HHK from different stages, the morphology and molecular biology change of HHK degradated in spinal cord was deeply researched. This study aimed to discuss the possibility of HHK lighten secondary lesion of spinal cord injury, improve metabolic microenvironment of neuron and nerve fiber, provide regeneration circumstances for neuron and nerve fiber. The main study contents, methods and results are as follow:1. Success established the experiment model of acute spinal cord injury of implanted HHK in rat in order producing a tissue-engineered spinal cord with HHK to repair SCI to provide normal animal experiment model.2. It was confirmation that HHK could promote tissue of injury spinal cord to produce factor of Anti-oxidation damage that reduced secondarylesion. The HHK changed differentiation and migration of proliferation neuroglial cell for avoided formation of necrosis cavum and glial scar after spinal cord injured and provide growth space of nerve regeneration.3. Behavior rehab of experimental group of implanted HHK was well than that of no implanted HHK.4. Suppression subtractive hybridization (SSH) was used to clone genes that are different between control and experiment groups on rat after 4th w. Using this SSH approach, amino acid transporter system A2 (Ata2), Cytohrome oxidaselll (COXIII), Peroxiredoxins 5 (Prd5), Tapl protein, cysteine proteinase inhibitor cystatin c (Cystatin C), were identified. Prd 5, Ata2 and COXIII played a role on reducing radicle, irritability amino acid, controlling calcium turbulence, and reducing other succeed damage to lighten the damage degree of spinal cord. Tapl protein and Cystatin C participated in dispelling HHK.5. The degradation mechanism of the HHK in tissue of spinal cord injury was detected. HHK began to degrade after 4th w under electron microscopy. Dispelling fragment of human hair keratin was form after 26th w. And the fragment disappeared all after 52nd w. There are two degradation ways. One of was ubiquitui peptidase system, the other was lyosomal enzyme system.Our research suggests that HHK can degrade in spinal cord, at the same time,stimulate local tissue to produce protect factors, which can lighten second lesion after spinal cord injury, and also can promote neuron regeneration and repair of spinal cord. This work gave a theory for using HHK as a tissue-engineered spinal cord.
Keywords/Search Tags:human hair keratin, spinal cord injury, rat, tissue engineering, tissue-engineered spinal cord, connector, morphology, praxiology, neuron, axis-cylinder, nerve fiber, myelin sheath, astrocyte, oligodendrocyte, microglia, ependymal cell, neural stem cells
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