| In recent years,microwave has been widely used in industry,medicine,communication,and other fields and it's biological action been investigated. Eye is one of the main target organs of microwave. Microwave has effects on visual system primarily involving cornea,lens and retina. It can induce opacity of lens,degeneration and necrosis of retinal neurons. The majority studies concentrated on the damage of lens induced by microwave before,but there are fewer reports on the damage of retina. This experiment was designed to observe the damage induced by 2450MHz (continue wave) microwave on cultured human retinal pigment epithelial cells and the protective effects of antioxidant zinc and glutathione on those damages of the cells. First time in this experiment,the transcription profile changes of genes related cells stress and apoptosis induced by microwave were investigated by microarray technique. And first time,the effects of antioxidants zinc and glutthione on the the transcription profile changes of genes related cells stress and apoptosis induced by microwave wereinvestigated by microarray technique. In order to further study the practiceapplication of antioxidant zinc in preventing the damage induced by the microwave,we firstly observed the protective effects of supplement zinc on the damage induced by microwave in the animal level. Our experiment data maybe can contribute to further study on the practice application of the prevention of zinc to the damage induced by microwave.Methods:1. To observe the effects of microwave on the cell morphology,biochemical indexes and proliferation of the cultured human retinal pigment epithelial cells According to microwave radiation intensity,the cultured hTERT-RPEl cells were divided into control group,10mW/cm2Xlhgroup,20mW/cm X lh group and 30 mW/cm X lh. The distance from the microwave source to cells was 16cm. After radiation,cell morphologies were observed under the optics microscope and transmission electricity microscope. The cell survival rates were measured by trypain blue staining. The activities of intracellular antioxidant enzyme SOD and GSH-Px and the content of intracellular oxidation products MDA were detected. Finally,the effects of microwave on the cell proliferation were observed by the cell culturing.2> To observe the transcription profile changes of genes related cells stress and apoptosis induced by microwave by microarray technique The temperature of the culture solution of the hTERT-RPEl cells was measured and recorded with the WDD-1 type full automatic thermometer during the microwave radiation of 10mW/cm2 for lh. The hTERT-RPEl cells were heated with the hot water to mimicry the heating effects of the microwave according the similar the rate and amplitude of the elevation of temperature. The cells of cultured hTERT-RPEl,heated by microwave and heated by water were collected. The mRNA extracted from the cells werelabeled by different fluorescent dyes with the reverse transcribing method. Two kinds of cDNA labeled were mixed and hybridized with the stress microarry Ars(200). The different transcription profiles of the genes were obtained by scanning the microarray with the fluorescent scanning bichromator. Tow sheet of microarraywere studied in this experiment,one was hTERT-RPEl cells vs the cells radiated by microwave,the other was the cells heated by water vs the cells radiated by microwave.3. To observe the protective effects of antioxidant zinc and GSH on the damage induced by microwave on the cell morphology,biochemical indexes and proliferation of the cultured hTERT-RPEl cells Toobserve the protective effects of zinc:The control group was the hTERT-RPEl cells radiated by 30mW/cm2 microwave for Ih. The protective groups were the cells cultured in the solution for 24h supplementing 0.1,0.2,0.5 mmol/L acetic zinc and radiated by30mW/cm2 microwave for Ih. To observe the protective effects of GSH:The control group was the hTERT-RPEl cells radiated by 30mW/cm2 microwave. The protective groups were the cells...
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