Affects And Mechanism Of Ginkgo Biloba Pingchan Recipe On The Apoptosis Of Dopaminergic Neurons In Parkinsonina Mice

Parkinson's disease(PD) is a neurodegenerative disease. It is one of themain diseases with high incidence and disable rate , also many sequelae. Amajor pathological hallmark of Parkinson's disease is a progressive loss ofdopaminergic neurons in the substantia nigra pars compacta(SNpc) and their axons,which project to the striatum. The loss of dopaminergic neurons results indecrease of dopamine in the striatum and imbalance in dopamine and acetylcholine.So Parkinson's disease is characterized by resting tremor ,rigidity and akinesia .Recent studies have suggested that main causes of neuronal death in PD areapoptosis. Despite extensive research, the disease remains poorly understoodand there is still no effective treatment. Chinese herbal medicine have efficacyat treating PD. According to the theory of traditional Chinese medicine and basedon clinical practices, Ginkgo Biloba Pingchan Recipe(GBPR) is consisted. Toelucidate the effect and mechanism of GBPR and its three separatedprescriptions, we observed the changes of apoptosis-related molecules andfactors in SNpc in Parkinsonian mice model with MPTP-treated. This study is consisted of four parts:(1)To observe the loss and apoptosisof dopaminergic neurons in SNpc in Parkinsonian mice and the roles of GBPR andits three separated prescriptions;(2) To observe the activity of themitochondrial complex in brain in Parkinsonian mice and influence of GBPR andits three separated prescriptions;(3) To observe the expression of thecalcium-binding protein calbindin D28k in brain in Parkinsonian mice andinfluence of GBPR and its three separated prescriptions by ABCimmunohistochemistry technique;(4) To observe the expression of the apoptoticregulated factors Caspase-9,Caspase-3,Bcl-2,Caspase-3mRNA and Bcl-2mRNA inSNpc in Parkinsonian mice and the effect of GBPR and its three separatedprescriptions by ABC immunohistochemistry and in situ hybridization technique. In experimental research, male C57BL/6J mice are injected intraperitoneallywith a total cumulative dose of 150mg/kg MPTP ,30mg /kg daily to induceParkinson's disease model. The modeled mice are called Parkinsonian mice. The英文摘要 5experimental animals is divided randomly into six groups:(1)The normal controlgroup( NC); (2) The model control group( MC);(3) Ginkgo Biloba Pingchan Recipegroup( GBPR); (4) Jiedu Recipe group (JDR); (5) Pinggan Recipe group(PGR); (6)Huayu Recipe group;(HYR) .The animals were killed respectively on the 14th andthe 28th day after first injecting with MPTP. The main results are displayed as follow: 1.Chinese herbal medicine Ginkgo Biloba Pingchan Recipe and its separatedRecipe may inhibit the loss and apoptosis of TH+ dopaminergic neurons In the experimental studying, ABC immunohistochemistry technique isused.The TH+ dopaminergic neurons were counted in every group . The number ofTH+ neurons in SNpc in MC is decreased by 52.14% on the 14th day and 60.78%on the 28th day.The option density for TH+ neurons and fibers also obviouslyreduced by 90.42% on the 14th day and 93.20% on the 28th day.The loss of TH+neurons are partly inhibited in SNpc of Chinese herbal medicine. The numberof TH+ neurons in SNpc of JDR and HYR is decreased by 16.6% and 17.55% on the14th day; The number of TH+neuronsin SNpc of GBPR and PGR is decreased by 22.72%and 20.61% on the 14th day. On 28th Day, The degree of loss of TH+ neurons in SNpcof GBPR is lightest ,for 33.2%; HYR,PGR and JDR are respectively for 43.94%,44.9%and 49.23%. The option density for TH+ neurons and fibers in SNpc of Chineseherbal medicine is also increased.The trend is similar to the changes of numberof TH+ neurons. The apoptotic cells in SNpc is found by Tunel tethnique. The number anddensity of apoptotic cells in SNpc in MC are increased,on 14th day is moreobvious. Chinese he...