| Background and purpose:Both morbidity and mortality of hepatic fibrosis, especially of cirrhosis, are quite high. So, it's very urgent to develop anti-fibrosis therapies against the hepatic fibrosis. Nowadays, the studies on hepatic fibrosis are being a worldwide hot medical subject. Because the hepatic-fibrosis or cirrhosis are induced mainly by chronic hepatic diseases, so their reversion is a difficult problem. Along with the emergence of effective anti-fibrosis therapies, reports of pathologically confirmed reversion or remission of the hepatic-fibrosis or cirrhosis have been increasing gradually. Western anti-fibrosis medicines, such as colchicines, have less satisfying effects but severe side effects, whereas the traditional Chinese medicines, as a great number of experiments approved, have effects to protect hepatic cells, improve hepatic functions, restrain activation and proliferation of hepatic stellate cells, inhibit secretion and activity of transforming growth factor-β1 (TGF-β1), accelerate degradation of extracellular matrix(ECM), etc., showing more predominance in promoting reversion of hepatic-fibrosis, and have been paid great attention by scholars domestic and abroad. In this study, female Sprague-Dawley(SD)rats were injected carbon tetrachloride subcutaneously to be as hepatic-fibrosis models, which were intervened by Ganfukang capsules(with three doses of high, medium, low), colchicines and Ganpikang(GPK)respectively. To explore possible mechanisms of the anti-hepatic-fibrosis effect of Ganfukang, conditions such as structure of hepatic lobules, degeneration of liver cells and inflammatory infiltration of the liver were observed under optical microscope; ultramicrostructure of hepatic cells was observed under electric microscope; sedimentation of matrix collagen fibers were observed and collagen component was analyzed under polarizing microscope; therapeutic effects were evaluated by determination of serous enzymes and hepatic functions; expressions of TGF-β1 and laminin(LN) were evaluated with immunohistochemical method. Genic expressions of matrix metalloproteinase(gelatinase-A/MMP-2)and tissue inhibitor of matrix metalloproteinase(TIMP-1)were determined by RT-PCR. Methods:Hepatic-fibrosis models were prepared by the means of subcutaneous injection of carbon tetrachloride(10%CCl4, 5ml/kg, 2 times a week for 13 weeks). SD rats were divided randomly into 7 groups: control group, model group, GFK high-dose group, GFK medium-dose group, GFK low-dose group, colchicines group, and GPK group. Drugs were given on the 60th day after models prepared. Animals of the groups mentioned above were poured normal saline, normal saline, GFK high-dose(3.125g/kg), GFK medium-dose(0.3125g/kg), GFK low-dose(0.03125g/kg), colchicines(1mg/kg)and GPK(0.55g/kg)respectively. The doses of drugs were counted according to the body area formula, 6.25 times the normal adult doses per kg body weight. The drugs were given, in solution of 10ml/kg, for 60 days. 24 hours after the last administration, blood were collected through inner canthus under ether anaesthesia to determine various indicators, and left lobes of livers were removed as samples for determination. Serum glutamic-pyruvic transaminase(GPT)and glutamic oxaloacetic transaminase(GOT) were determined by Wright-Giemsa's staining, serum albumin, total protein and the ratio of albumin and globulin were determined by bromocresol green colorimetry and Coomassie brilliant blue colorimetry, serum hydroxyproline(Hyp)and sialic acid(SA)by colorimetry, Hyp in liver tissue by digestive method, LN and TGF-β1 by immunohistochemical method. Structures of liver lobules, degeneration and inflammatory infiltration were observed under optical microscope, ultramicrostructures of hepatic cells were observed under electric microscope. The sedimentation of matrix collagen fibers stained by picrosirius-red were observed under polarizing microscope. Collagen areas of type I and III and their ratio were analyzed by Image-Pro Image Analyzing System. Genic expressions of MM...
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