Experimental Study On Labeling With Radioactive Nuclide And SLN Particular Localizaton Of Flt4 Antibody

Objective: To research a novel method of sentinel lymph node(SLN) localization. Onprecondition that Flt4PcAb is a particular molecular marker of lymphatic endothelium, tolabel Flt4PcAb with radioactive nuclide and detect γ radial in order to study feasibility ofSLN localization with Flt4PcAb. We expect to guide-localize SLN by mean of immune effectbetween receptor and ligand and further provide experimental basis of tongue carcinoma'sSLN localization accurately. Methods: 1. To label Flt4PcAb with 125I via chloramine method and detecte biological andsafe trait of125 I- Flt4PcAb. 2. To analyse lymph node guide-localization ability of 125I-Flt4PcAb and study the metabolic kinetics of 125I- Flt4PcAb; To detect SLN of the transplanttumor on BALB/C mice with Tca-8113 human tongue carinoma by 125I-Flt4PcAb.3. To labelFlt4PcAb with 99m Tc in order to search for optimal condition. 4.To study feasibility of lymphnode guide-localization with 99mTc—Flt4PcAb and compare the results between99mTc—Flt4PcAb group and 125I—Flt4PcAb group. Results: 1.Average labeling efficiency was 49.4% and the radiochemical purity of 125I-Flt4PcAb was 95.7% after purified. 125 I—Flt4PcAb was stable on the condition of 40Cand negative bacterium and heat source. 2. The ID(%)/g of popliteal lymph node washighest and blood higher and the other organs low 30 minutes after 125I- Flt4PcAbsubcutaneous injection at dorsum of foot. The ratio of ID(%)/g (popliteal lymph node to blood)was greater than 1.5 within 16h after 125I—Flt4PcAb injection. 125I- Flt4PcAb metabolismafter subcutaneous injection met a two compartment fitted model. The mean value of T1/2kaand T1/2β(x±s) were 0.62±0.09d and 3.51±0.47d respectively. 3. The rate of transplanttumor were 100 percent. In the 70 nude mice, micrometastasis of tumor were detected in threecases of 70 experimental lymph nodes by HE-stained method and the result was the samewith immunohistochemical method in these three cases. Besieds, in the rest 67 experimentallymph nodes, two cases were found micrometastasis of tumor by immunohistochemical 5第二军医大学 英文摘要 博士学位论文method. Statistical analyses showed that there were significant difference of 125I-Flt4PcAbuptake among normal hyperplastic and burden with tumor lymph nodes and for the burdenwith tumor lymph nodes it was lowest and for the normal lymph nodes it was highest. 4.About labeling Flt4PcAb with 99mTc, label efficiency raised along with time postponementand was stable at the point of 30 minutes after action. Raise of label efficiency was alsobenefit from raise of temperature, but it did not raise prominently when it was more than400C. Besides, label efficiency was higher on condition of high Sncl2 concentration than lowSncl2 concentration and it was stable when Sncl2 concentration was more than 0.875μg/ml.Label efficiency was higher when PH value was between 5.5 and 9.5. Stabilization in vitro ofFlt4PcAb labled with 99m Tc was good. 5. Radioactivity intake of popliteal lymph node washigher in 99m Tc-Flt4PcAb group than 99m Tc-control group(P<0.01) and residualradioactivity on injection position was lower in 99mTc-Flt4PcAb group than 99m Tc-controlgroup(P<0.05).Compared to 125I-Flt4PcAb group, radioactivity intake of popliteal lymphnode and residual radioactivity on injection position were low in 99mTc-Flt4PcAbgroup(P<0.01). Conclusion: 1. Flt4PcAb can be labled with 125I via chloramine T method successfully.125I- Flt4PcAb was fit for animal experiment after purified. 2. 125I-Flt4PcAb was a potentialagent for lymph node guide-localization. Its metabolism met a two compartment model...