| For several decades, the researchers of all around the world had done great hard to reveal the essence of kidney deficiency syndrome, and had tentatively confirmed that there are some modern pathophysiological basis. The details of some traditional Chinese medicine theory and its science nature had been partly explained, and some objective marker had been found. In these researches, there are many studies on the kidney yang deficiency syndrome, while little study on the kidney yin deficiency syndrome. Though there are many achievements on the study of kidney deficiency syndrome, some puzzle also remains. The Chinese medicine "syndrome" is the summarize of pathology and physiology of the different phases of different diseases, and since there are some common clinic manifestation and phathomechanism, there must be some common material basis. According to the life central dogma, the genetype (DNA or gene) is the most primary and the most essential matter of the life, and master and confine the phenotype program (protein to cell to organism). Thus, the life phenomena, diseases, Chinese medicine syndrome can all have the most essential trace on the DNA or gene. The traditional Chinese medicine considers that the kidney stores the spirit, and is the congenital foundation, and charges the development, growth, reproduction and fluid metabolism. The spirit in the kidney comes from the essential substance of reproduction from the parents, and is the primary substance for embryonic development. So, it serves the common identity with the genome DNA. The material basis of kidney deficiency syndrome is the kidney spirit insufficiency. Many domestic professors think that there are a lot similarity between the methodology of human genomic study and traditional Chinese medicine's whole discrimination, the genomics would be a great breakthrough of the research on kidney deficiency syndrome. Therefore, to research the molecular mechanisms of kidney deficiency syndrome from the genome has a great significance.Diabetic nephropathy (DN) is a common disease which harms people-5-greatly. And sub-health has become the the most important invisible killer to the health and difficult problem to the modern medicine nowadays, sub-health is the transition state between illness and healthy, which has a close relationship with the Chinese medicine syndrome. We found the DN and sub-health can show up the kidney yin deficiency syndrome, and the former is the kidney yin deficiency syndrome of the illness state, the latter is the kidney yin deficiency syndrome of the functional state. The homotherapy for heteropathy must have the same material basis, therefore, it would be very perfect to select DN and sub-health to be the study objects of the related genes of the kidney yin deficiency syndrome.OBJECTIVE:To research on the related genes of kidney yin deficiency syndrome from structural genomics and functional genomics.METHODS:1. suppression subtractive hybridization(SSH) was used to obtain differentially genes associated with kidney yin deficiency syndrome of DN and Sub-health, comparing blood DNA of patients with kidney yin deficiency of DN and sub-health (as Tester or Driver) with normal blood DNA (as Tester or Driver). After Rsa I enzyme restriction, Tester DNA were divided two groups to ligated to the specific adaptor 1 and adaptor 2.Then Tester DNA were hybridized with Driver DNA twice and underwent nested PCR twice. The PCR product was ligated with U plasmid vectors, transformed into E. coli TOPIC, screened through the blue-white screening system, and then positive recombinant clones were confirmed by PCR method. After that, we farther screened the positive clones using the dot blot technology. We select the positive clones from the DNA subtractive library to go to sequencing and homology analysis.2. Using the gene chip technology, we study the gene expression spectrum of kidney yin deficiency syndrome of sub-health. At the same time, we study the pharmacodynamic action genomics of the Chinese famous prescription Liu-We...
|
PDF Full Text Request