| 1. A type of wound aerosol dressing (WAD) is to be developed, which is small in size and portable. It can be used easily by non-medical personnel, especially those with little knowledge of first aid, and used on wounds either on the battlefield or in pre-hospital rescue. Immediately after the WAD is prayed onto the wounds, bleeding is stopped and a bacteriostatic effect of the WAD is achieved. The main aim is to provide a substitute of tourniquet and first-aid sterilizing dressing such as gauze if possible, in order to prevent shock caused by heavy bleeding and rescue the injured limbs and reduce the chance of infection.2. The WAD can quickly and effectively stop bleeding in various oozing wounds, and wounds with main artery ruptures of the limbs.3. The WAD has a bacteriostatic effect on some pyogenic gram-positive and -negative and epiphyte.4. The WAD can be proved to be safe and reliable by biological evaluation test system.Methods1. Criteria for research and development of the WAD are to be establishedaccording to its intended use and each ingredient is selected accordingly, andthe formula is ultimately determined by conducting chemical experiments.2. 33 models of blood oozing wounds of swines, including concave wounds, lateral wounds in swine hips and wounds across knee joints, are established by means of cutting injury. After injury, the WAD is sprayed unto each wound model within 5 to 10 seconds. The main indexes, including average arterial blood pressure (AABP), heart rate (HR), hemostatic time (HT), the formation time and the thickness of WAD membrane shaped after spraying, are measured and the WAD membrane is observed to see if it raptures within 12 hours postoperatively. Examinations of histology and both of TEM and SEM are also performed in three concave wounds models in 0.5h, 1.0h, 2.0h, 3.0h, 4.0h, 6h, 8h, and 12h.3. By means of cutting injury, 60 bleeding wound models with partial raptures of the femoral artery in 60 swines are established according to the following conditions before application of the WAD: (1) whether hemostasis by finger pressing or tourniquet has been applied; (2) whether the blood is cleaned. 6 categories of hemostatic models are established, including tourniquet, pressing and direct WAD spraying hemostasis onto cleaned and uncleaned wounds. Each category consisted of 10 swine models. The main indexes for observation and measurement within 12 hours postoperatively involve average arterial blood pressure (AABP), heart rate (HR), hemostatic time (HT), the formation time and the thickness of the WAD membrane, and possible raptures and succeeding bleeding. The success rate of each method is calculated.4. Experiments are conducted on contaminated wounds to see the bacteriostatic effect of the WAD. 13 round wounds with a diameter of 2.5 cmand as deep as the muscle layer are performed on both sides of the backs of 6 swines. 3 10CFU/ml Staphylococcus aureus solution of are laid on each wound. 15 minutes later, one wound in each swine is chosen as blank control and a small specimen is taken for bacterial counts. The other 12 wounds in each swine are divided evenly into test groups (spraying the WAD to each wound) and control groups (covering the wounds with sterilized gauze directly). 1h, 4h, 8h, 12h, 18h, and 24h after covering the wounds, one wound is chosen from each group for observation and small specimens are taken from them for bacterial counts.5. Such Pathogenic microorganisms as Staphylococcus aureus, Staphylococcus citreus, hemolytic streptococcus, Staphylococcus epidermidis, escherichia coli, bacillus perfringens, bacillus proteus vulgaris, pseudomonas pyocyanea, avirulent bacllus anthracis, avirulent bacillus pestis, bacteroides fragilis and Candida albicans are revivified and inoculated into the culture media respectively. Sterilized filter papers with a diameter of 6mm are placed in the center of each culture medium. The WAD is dropped in the dosage of 10 1 per paper and the culture media are cultured...
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