Effect Of The Shenluotong On The Renal Tubular Epithelial-myofibroblast Transdifferentiation

Objective:Chronic renal failure is a chronic progressive disease andneed the effective medicine to protect renal function. The traditionalChinese herbs are used in clinic for long time and its good result wasrealized, especially at the early stage. Shenluotong was an effectivemedicine, which was created by professor Zhao Yuyong according totraditional Chinese medicine theory combining the renal pathologic andpharmacological study used for kidney diseases for many years in clinic.The drug was composed of membranous Acuiellaria baicalensis, Salviamiltiorrhiza, Ligusticum wallichii, Bombyx batryticatus, Zaocys andRadix et Rhizoma Rhei, etc. It has the functions of promoting circulationof qi and the blood, dispersing blood stasis and dredging collateral. Theprevious primary experimental study and clinic data showed that theprescription had good effect to against glomerulosclerosis, and renaltubule interstitial fibrosis. To further study the effect of Shenluotong onthe expression ofα-SMA in renal tubular epithelial cell and regulativefactors, light microscopy, electron microscopy, radioimmunoassay,immunohistochemical method, in situ hybridization, flow cytometry andpathologic image pattern analysis were used in the test. To observe the24h total urinary protein, serum creatinine, blood urea nitrogen, NAG andβ2-MG in urine, serumβ2-MG, pathomorphological changes of renaltissue. The expression ofα-SMA, renin activity, angiotensin II, TGF-β1,TGF-β1mRNA, c-fos, extracellular matrix FN and LN were examined.The study is just for researching the mechanism of Shenluotong.Part I Effect of Shenluotong on the renal function andpathomorphology of the rats induced by amycin 9英 文 摘 要Methods: 45 healthy SD rats, 170±10g weight, were fed for one week toadapt the environment. After the urinary protein was tested to be negative,the rats were randomly divided into sham operation group, Model group,Shenluotong group and Valsartan group. The Model group were made byunilateralnephrectomy with twice-amycin injections by caudal vein. Theexperimental period was lasted for 10 weeks. The blood was got and keptin cold storage forβ2-MG, creatinine, BUN and so on. Left kidney washarvested to weigh. The renal tissues were fixed for pathomorphology,HE, Mosson and PAS staining for light microscopy. The samples forelectron microscope were fixed with routine method, then dehydrated,embedded, sectioned, double colored. The ultramicro structures of renalproximal convoluted tubule and distal convoluted tubule were observedwith electron microscopy.Results:1 Kidney weight / body weight and the data of 24h urinary proteinThe body weight of model group increased slowly after the secondinjection and even lost. The worse hair color, heavy proteinuria and theexpanded kidney were showed at the end of test. The 24h total urinaryprotein were 28.85±10.68mg/24h (sham group), 421.06±152.7mg/24h(Model group), 297.96±36.24mg/24h (Shenluotong group) and 303.3±89.96mg/24h (Valsartan group) respectively. The data of Model groupwere higher than sham operation group significantly (p<0.01);Shenluotong group and Valsartan group were lower than that of Modelgroup significantly (p<0.05); The effect of Shenluotong group superior tothat of Valsartan group, but that had no statistics meaning. The index ofkidney weight / body weight were 0.37±0.04 (sham operation group),1.40±0.18 (Model group), 1.10±0.09 (Shenluotong group) and 1.31±0.34 (Valsartan group) respectively. The Model group was higher thanthat of sham operation group significantly (p<0.01); Shenluotong groupwas lower than that of Model group significantly (p<0.05); There was nosignificant difference between Shenluotong group and Model group. 10英 文 摘 要2 The results of serum creatinine, urea nitrogen, urinary NAG,blood β2-MG, urinaryβ2-MGThe serum creatinine were 128.2±12.10μmol/l (sham group...