Studies On The Characteristics Of Traditional Chinese Medical Syndrome Of Alcoholic Liver Fibrorosis And On The Molecular Mechanisms Of Manganxiao Compound Anti-Fibrorosis

Alcoholic liver disease is a kind of common disease in clinic. Alcoholic fibrosis isthe most phase on the way transforming from alcoholic fatty, alcoholic hepatitis toalcoholic liver cirrhosis. As its reversibility, alcoholic fibrosis has been recognized asone of the most exciting domains in the studies on the forming mechanisms ofalcoholic liver fibrosis. The present studies partly concentrate on the activation ofhepatic stellate cell and excessive excretion of type I collagen. On the grounds oftechniques of Cytobiology and Molecular Biology, we aim at the research in thecellular and molecular mechanisms of Manganxiao compound anti-AF(alcoholic liverfibrosis) . Traditional Chinese Medical therapy has potential superiority and distinctcharacteristics in the treatment of alcoholic liver fibrosis. By means of the methods anddoctrines of Clinical Epidemiological Research, we aim at the investigation of thecharacteristics of traditional Chinese medical(TCM) syndrome of alcoholic liverfibrosis, attempt to explore a better way for the treatment of AF. In the theoretical part , I summarized the cognition of the cause and mechanismsof alcoholic liver fibrosis by the angle of traditional Chinese medicine; discoursed atgreat length on the Mentor TianDelu's scientific thought; summarized succeedly thedevelopment of the AF treatment by the way of Chinese traditional medicalcompounds and effective components, and of the clinical epidemiology in the inlandstudies on alcoholic liver fibrosis. On the other hand, expounded systemically thepathogenesis of alcoholic liver fibrosis by the angle of western medicine. In the clinical investigation, our group studied clinical cases in multi-centerprospective method. In virtue of Factor Analysis, we explored the characteristics oftraditional Chinese medical syndrome of alcoholic liver fibrosis, and the formingcharacteristics of alcoholic liver fibrosis in inland. In the experimental research , cellar culture was mainly taken, rat hepatic stellatecells(HSC-T6) were regarded as the study object, acetaldehyde was used as thestimulating factor, Manganxiao compound was the experimental drug. On the groundof the methods of MTT and Serum Pharmacology,my group analyzed the effect ofManganxiao compound on the proliferation of HSC-T6, In virtue of the methods ofRadioisotope, we quantitatively determined the activity of rat α1(I) collagen genepromoter, and analyzed the effect of Manganxiao compound on the activity. In result,we partly opened out the molecular mechanisms of Manganxiao compound6 酒精性肝纤维化中医证候特点和慢肝消抗酒精性肝纤维化的分子机制anti-AF(alcoholic liver fibrosis) and its role target. Result and Conclusions (1) clinical investigation Experiment one: Male was the major case among the patients of AF, the mean ageof AF patients was approximate to 45a. The incidence of AF was different with thekinds of profession, the most was officers(44.57%), the second was businessman(23.91%); The prevalence rate of AF was relatively higher when the drinkersconsumed the equivalent of 140g per day with a mean duration of 20 years, which wasapproximate to a total alcoholic consumption of 1000kg. With increase of alcoholicconsumption, The incidence of the serum levels of γ-GT, or ALP(>110 U/L), orAST/ALT(≤2) increased. Experiment two: Among the subjects, the characteristics of TCM syndrome ofalcoholic liver fibrosis mainly manifested excess syndrome with the commonmanifestations of deficiency and excess; The visceral position was mainly in liver andspleen, related to gallbladder and stomach; The result suggested that stagnation of qi ,blood stasis, damp heat, with deficiency of genuine qi were the interpretation of thecauses of alcoholic liver fibrosis. The total alcoholic consumption was one of theimportant factors which effected on the distribution of the characteristics of TCMsyndrome of alcoholic liver fibrosis, so was the total alcoholic consumption wh...