Comparative Proteomic Analysis Of ACC Cell Tumor Metastasis Associated And P19 Cell Neuronal Differentiation-associated Proteins

Metastasis is the most insidious and life threatening aspect of cancers. Howeverlittle is known about the molecular mechanisms of tumor metastasis. Adenoid cysticcarcinoma (ACC) has a high incidence of distant metastasis (DM) to lung, which isresponsible for a rather low long-term survival rate. In the present study, a poorlymetastatic Acc-2 cell line and a highly metastatic Acc-M cell line were selected as anexperimental model to study the mechanism of metastasis. Two dimensional gelelectrophoresis and mass spectrometry are combined to approach the protein profilesassociated with tumor metastasis between Acc-2 and Acc-M cell lines. Comparativeproteomic analysis revealed that 12 protein spots showed significantly variations. Ofthe identified proteins, transketolase, Dim1p, v-Ha-ras oncogene, type I collagen proalpha, tumor necrosis factor (ligand) superfamily member 4, and pirin etc, have shownsome associations with distinct aspect of tumor metastasis. The different expressionpatterns of these 12 spots suggest the different roles they may play in tumormetastasis. However, further studies are still needed to elucidate the real roles of thesedifferentially expressed proteins. Our work is an initial step towards uncovering themolecular mechanism of tumor metastasis, and may contribute to discover diagnosticmarkers and therapeutic targets.Interests have been growing over the past years to understand the molecularmechanisms of neurogenesis. However, because of the complexity of the neuralsystem and the inaccessibility for experimental manipulation, little is known aboutthis system. The mouse P19 embryonic carcinoma cell is a well-documented system...