Establishment Of An Immortalized Human Fetal Hepatocyte Cell Line And Exparimental Study Of Hepatocytes Transplantation

As a severe threaten to people's health end-stage liver diseases, including hepatic carcinoma, decompasate liver cirrhosis and hepatic function failure, are still very difficult to cure in clinical practice by traditionary regimen or medicine. Recent years liver transplantation has become the most effective therapy for end-stage liver diseases. However, because of the shortage of organs available for transplantation and the parallel growth of the transplant waiting list, many patients still are dying without having a transplant choice.Because of the ability of the liver to regenerate through hepatocyte proliferation, many researchers have proposed and investigated the use of isolated liver parenchymal cells for the treatment of several liver diseases. One of the possible clinical applications is hepatocytes transplantation. This method could not only support, but also be an alternative to liver transplantation sometimes, allowing more patients to be treated. Moreover, hepatocytes transplantation potentially could present several advantages over whole organ or partial liver transplantation, such as less invasiveness, possibility of repetition and scheduling, no need for chronic immunosuppression, and, finally, the transplantation of more than one recipient from a single donor.The base for application of this therapy is the availability of a sufficient amount of liver cells. Many sources of human liver cells have been proposed and investigated, from tumoral cell lines to primary animal hepatocytes and, recently, hepatocytes from bone marrow and hepatic derived progenitor liver cells. The aim of this study is to construct an immortalized human fetal hepatocyte cell line, and to study its functional characteristics after transplantation, for the development of a nationally cell material in hepatocytes transplantation. The results are as followings:1 The recombinant plasmid pcDNA3.1/SV40T was constructed with eukaryotic expression vector pcDNA3.1 and simian virus 40 large T antigen gene using standard method. The construction of recombinant plasmid was identified by...