Experimental Study Of Neuroprotective Effects Of TMP And FK5 On Acute Spinal Cord Injury In Dog

Objective To explore the neuroprotective effects of tetramethypyrazine (TMP)and FK506 and study change and significance of S-100B and myelin basic protein in serum and cerebrospinal fluid on acute spinal cord injury in dog . Methods By striking the spinal cord at the level of T13 in dog with Allen's device ,Acute spinal cord injury was induced in anesthetized dogs . Animals received high selectivity arterial duct intubation. Animals were randomly divided into the blank group,the control group; the higher,lower dosage tetramethypyrazine treatment group and the higher,the Lower dosage fk506 treatment groups. MRI was performed 30min,24h,72h,1w after injury. Neurological status was assessed by the Xu shao-ting scoring system after injury, and spinal cords were harvested for histopathology observation . Immunohistochemical technique and image analysis were used to examine the expression of NF in neurons,GFAP in astrocytes. some dogs were taken blood and CSF after the operation. The concentration of MBP and S-100B in the serum and CSF was measured by the methods of ELISA. Results the higher dosage tetramethypyrazine and fk506-treated dogs had significantly better neurology outcome compared with the control group and lower dosage group . Histopathologic analysis disclosed severe injury in the spinal cord tissue of all control dogs compared with the higher dosage tetramethypyrazine and fk506-treatment group. By the observation on the immunohistochemical slices and image analysis by the Image-pro plus image analysis system. we find that the higher tetramethypyrazine and fk506-treated dogs had less injury than the control group and the lower group. Tetramethypyrazine and fk506 significantly increased the expression of the NF in neuron and GFAP in the astrocytes. Concentration of MBP and S-100B in the serum and CSF increased significantly in control group and tetramethypyrazine Objective To study the change and significance of myelin basic protein and S-100B protein in serum and cerebrospinal fluid(CSF) after acute spinal cord injury(ASCI) with tetramethypyrazine treatment Methods Dog models with acute spinal cord injury in T13 were made by the methods of Allen's and an epidural tube was set in the subarachnoid space .The tube was drawn out of the skin and fixed in order to take the CSF. The dogs were divided randomly into three groups. Group A (n=6) was the control group, which received the operation except the striking to the spinal cord. Group B (n=6), acute spinal cord injury group and Group C (n=6) ,tetramethypyrazine treatment group,were the same group that received the striking to the spinal cord with the devices of Allen's after the dural was exposed. These dogs were taken blood and CSF at the time of 2h, 4h, 6h, 8h, 10h, 24h, 48h, 72h and 96h after the operation. The concentration of MBP and S-100B in the serum and CSF was measured by the methods of ELISA and features of the change were studied. Results Levels of MBP and S-100B in the serum and CSF increased significantly in group B and C group, and the levels changed dynamically. But the levels of group C is lower than that of group B. Conclusion MBP and S-100B protein changed dynamically after acute spinal cord injury, tetramethypyrazine have treatment effect for acute spinal cord injury, MBP and S-100B protein can be seen as the marker for ASCI and can be further studied to evaluate their correlation to the severity and prognosis of ASCI.