Expression Characteristic Of Three Hsps Gene Families During Normal Organs Development And During Short-limb And Cleft Palate Development In ICR Mouse

Expression characteristic of three Hsps gene families during normal organs development and during short-limb and cleft palate development in ICR mouseAbstract In this study, the characteristic of three heat shock proteins genes (Hsps), which were small heat shock proteins (sHsps), Hsp40s and Hsp60s, was systematically studied during ICR mouse normal organs development. Using the model of cleft palate, short limb and Oligodactyly, the characteristic of Hsps genes in the abnormal limb and palate were studied, and then the Hsps with special change in the model were selected, their function in the embryo development was studied by combined use of RNA interference (RNAi) strategies with in vitro limb bud culture and whole embryo culture (WEC) model systems. We elucidated the characteristic of the three Hsps gene families during mouse normal organs development, and their possible roles in cleft palate and short limb. Combined use of RNAi strategies with in vitro mouse limb bud culture and mouse post-implantation WEC model systems were established initially. So, the base of theory and methods that studied the function of Hsps in normal and abnormal development was established.