Experimental Studies On Treatment Of Liver Cancer With Double-Regulated Replicative Adenovirus CNHK500-hγ Carrying Human IFN-γ Gene

CNHK500-hγ, a novel oncolytic adenovirus carring hIFN-γ with its essential gene doubly driven by hTERT and HRE promoter was constructed in the present study, and its anti-tumor efficacy on HCC both in vitro and in vivo was investigated.Conclusions CNHK500-hγ are capable of replicating in and lysing HCC cells selectively. The amplification of the therapeutic gene h y expression is accompanied with replicating and proliferating of CNHK500-hγ in HCC cells both in vitro and in vivo. The tumor growth inhibition of CNHK500-h Y is superior to that of ONYX-15 and Ad-h γ, suggestive of synergistic effects of the oncolytic virus vector and the therapeutic gene. Being capable of replicating in and lysing HCC cells selectively with potent tumor growth inhibition in vivo, both of CNHK500-hγ hold good potential for the treatment of HCC.