Mechanism With Evaluation Of Neuroprotective Effect Of Salvianolic Acid A

Neuroscience is focused on such problems as neuroprotective, rehabilitation, loweringhandicapped rate. Now a lot of drugs are not able to reduce handicapped rate. "9-5" or"10 5" results show Chinese herbs formula and some component have advantage overcerebral lenisons.Aim:To evaluate impersonal neuroprotective effect of Salvianolic acid A on cerebralischemia; To discuss neuroprotective action mechanism and pathway and relativetarget; To explain neuroprotective action character of Chinese herbs.Methods:The whole study has integrated 2 kinds of appraisement means(In vivo and invitro),Copied MCAO and established Hypoxia-Reoxygen cellular model. On onehand, evaluate protective effect of SalA on acute cerebral ischemia lensions by suchindexes as nerve function grade, cerebral infarction index, cerebral water content,cerebral tissue morphologic(HE); On the other hand, appraised successively effect ofSalA on Hypoxic-Reoxygen neuron or astrocyte cell by such technologies as flowcytometry(FCM), ELISA, RT-PCR, etc. The indexes includes MTT, LDH, Cellviability, apoptosis rate, cell ultrastructure, NSE, NGF, IL-lp and so on. Then we haveprepared 4 kinds of astrocyte medium(AstM) and compared or Validated their actionone another along with SalA. At last, we have initially discussed possible pathwayand relative target of SalA.Results:1. SalA can improve nerve behavior defect and reduce cerebral infarction index and cerebral edema obviously. The low-dose group(2.5mg/kg) is the best of 3 concentrations; Effectiveness dose not enhance along with increase of dose.2. The effect of SalA is more powerful than SalB on some main indexes of cerebral ischemia.