| Objective: 1.To get acquainted with the operation of ligating the anterior descending branch of coronary artery of rats to form myocardial infarction models. To analyze the availability and the results of invasive method in heart function detection with acute myocardial infarction. To investigate the expression of VEGF receptors(Flt-1, Flk-1/KDR) and receptors(Tie-2) of myocardium in myocardial infarction group. 2. To investigate the angiogenesis of myocardium in the 2nd, 4th, and 6th week after acute myocardial infarction and the expression of VEGF receptors(Flt-1, Flk-1/KDR) and angiopoietin receptors(Tie-2), as well as their mRNA, of myocardium. To analyze the intragastric pre-administration with Rhodiola Rosae or Dalbergia odorifera on the angiogenesis of myocardium, and on the expression of VEGF receptors(Flt-1, Flk-1/KDR) and angiopoietin receptors(Tie-2), as well as Flk-1/KDR and Tie-2mRNA, of myocardium. To discuss the effectiveness, the significance and the probable mechanism of Chinese traditional medicine on the angiogenesis. 3. To observe the changes of heart function of rats in the 2nd, 4th, and 6th week after acute myocardial infarction, as well as the changes of heart function of rats intragastric administration with decoction of Rhodiola Rosae or Dalbergia. 4. To investigate the effect of captopril on the angiogenesis of ischemia myocardium and on the expression of Flk-1/KDRmRNA and angiopoietin receptors(Tie-2)mRNA. Through those experiments, the experimental methodology of animals with acute myocardium ischemia was mastered. A blood vessel neogenetic regulating system with VEGF receptors(Flt-1, Flk-1/KDR) and angiopoietin receptors(Tie-2) was built-up to analyze the changes of the system while myocardium ischemia set on and the results and their explanations while being intervened with Chinese traditional medicine. Some theories on the mechanism and the significance of the angiogenesis may be advanced.Methods: 1.Build animal models: Perform thoracic operations on SD rat, ligating the anterior descending branch of coronary artery of the rat. 2. Preparation of the decoction of Chinese traditional medicine and grouping: Decoct the original medicine together with distilled water, and filtrate the gruffs with sterile gauze. Decoct the gruffs for the second time and filtrate. Mix these two solutions to make the decoction (the concentration was 0.5g/ml). The dose of intragastric administration was 3g/kg, which was 2ml/300g (per rat). The rats were divided into model group,Rhodiola Rosae group, Rhodiola Rosae pre-administration group ( pre-administration Rhodiola Rosae for 7 days before the operation), Dalbergia odorifera group, captopril group, pseudo operation group, non-intervened group, and positive control group. 3. Detection of the heart function: Separate the right cartid artery of the rat, put catheter through it to the ventricle. Detect the parameters by pressure converter and analyze the dp/dtmax, LVP, LVEDP with Polygragh system and Apple computer. 4. Detect the expression of factor VIII, PCNA, VEGF receptors(Flt-l , Flk-1/KDR) and angiopoietin receptors(Tie-2) in myocardium at the edge of the myocardial infarction part by immunohistochemical methods. The positive area and positive OD of the slice were analyzed by IMS image analysis instruments and related software. 5. Fluorescence quantification PCR (FQ-PCR) was used to detect the copies of mRN A of VEGF receptors (Flk-1/KDR) and angiopoietin receptors (Tie-2). 6. Detect the expression of CD34 in myocardium by fluorescence immunohistochemical methods. 7. The results were express with *±S, and the difference among the groups was tested by q test (multiple comparisons of one-factor analysis of variance). SPSS 10.0 was used in the data processing.Results: In the first part, the successful rate of model making was 45%. The heart function detection: In the 6th week after MI, the dp/dtmax value of model group, positive control group, pseudo operation group, and non-intervened group was 2615 ±112.4, 2733.2 + 177.5, 3965 + 467.2, and 4369.4 + 390.3 respectively. Compare model group with the other group p<0.05. On the 6th week, the positive area of VEGF receptors(Flt-l, Flk-1/KDR) and angiopoietin receptors(Tie-2) of the myocardium was 20399.50±6614.3, 28645.58±11103.8, and 20885.58±5891.0 respectively. Compare model group with the other groups on the expression of Flk-1/KDR: to pseudo operation group and non-intervened group p<0.05, to positive control group p>0.05, while compared on the expression of the other two receptors, all p>0.05. In the second part, the dp/dtmax value of model group in the 2nd, 4th, and 6th weeks was 2565.0±98.1, 1617.1±902.3, and 2089.2±288.8 respectively; Rhodiola Rosae group was 2826.2±267.2, 2730.0±334.7, 2699.2±241.4 respectively; Dalbergia odorifera group was 2230.6±596.3, 2028.1±288.8, 2567.5±91.1 respectively; Rhodiola Rosae pre-administration group was 3865.0±997.8, 3034.1±391.6, 3190.6±372.9 respectively; and the non-intervened group was 4143.7±106.2 > 4455.0±397.1 -. 4316.8±260.2 respectively. Compare the difference among the groups, p<0.05. If take the dp/dtmax value of non-intervened group as basic value, comparethe difference of the results of the dp/dtmax value of other groups minus basic value, p<0.05. In the second part, in the 6th week, the results of the expression of factor VIII tested by immunohistochemical methods of model group, Rhodiola Rosae group, Dalbergia odorifera group, Rhodiola Rosae pre-administration group, pseudo operation group, and non-intervened group was 11810.2±2533.6, 41535.7±5870.3, 37214.3±1044.7, 38558.7±7225.0, 22092.1±5779.9, and 16692.5±5778.9 respectively. Compare the difference between each two groups, there was no difference (p>0.05) between Rhodiola Rosae group, Dalbergia odorifera group and Rhodiola Rosae pre-administration group, while compare them with model group, pseudo operation group and non-intervened group, the expression was increased, p<0.05. The positive area of the expression of PCNA of above groups was 15033.5±4178.9, 32179.7±2249.7, 32784.0±9262.4, 41202.2±9615.0, 21184.6±6495.1 and 16391.2±6134.2 respectively. Compare the difference between each two groups, the positive area of Rhodiola Rosae group, Dalbergia odorifera group and Rhodiola Rosae pre-administration group was bigger than model group, pseudo operation group and non-intervened group (p<0.05). The quantification result of the expression of CD34 in myocardium tested by immunohistochemical methods of the above groups in the 2nd week was 52.6 + 22.1, 78.7 + 22.9, 39.0 + 20.9, 76.3±26.4, 56.2+14.6 and 57.5 + 25.8 respectively. The result of Rhodiola Rosae group and Rhodiola Rosae pre-administration group was higher than the other groups, p<0.05; in the 4th week was 40.7+17.8, 63.8 + 25.0, 44.3 + 24.7, 87.3+25.1, 46.7±24.4 and 21.2±7.9 respectively. The result of Rhodiola Rosae pre-administration group was increased significantly (p<0.05), while there was no significant difference between the other groups (p>0.05). The quantity of mRNA of Flk-1/KDR tested by FQ-PCR in the 2nd, 4th and 6th week of Rhodiola Rosae pre-administration group was 954. 2 +135. 6, 808. 3 + 93. 9, 781. 3 + 99. 7 respectively; The copies of mRNAof Tie-2 was 3694. 2 + 362. 5.2866. 8 + 308. 7.3157. 1+258. 2 respectively, which was much higher than the other groups at the same time, pO.OOl. In the third part, the density of capillary vessel of myocardium on the edge of the myocardial infarction part of captopril group in the 2nd and 4th week was 265.6 + 45.4 and 347.5±39.4 respectively. Compare with model group, p>0.05, and with Rhodiola Rosae group, p<0.05; The copies of mRNA of Flk-1/KDR tested by FQ-PCR of captopril group in the 2nd and 4th week was 413. 3 + 35. 7 and 377. 6 + 63. 0 respectively, which was significantly lower than Rhodiola Rosae group (pO.Ol), model group and non-intervened group (p<0.05);...
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