The Research On Function Of The OFC In The Mechanism Of Morphine Dependence

Objective: To establish models of morphine dependent rats with conditional place preference(CPP) method research the effects of the DA D2 receptor on morphine dependence rat by behavioral methods(namely CPP) .To discuss the effects of the DA D2 receptor in the orbital frontal cortex in rat associated with the processing of opioid dependence. To hope to provide a theoretical rational mechanism between the DA system in orbital frontal cortex and drugs addiction.Methods: CPP models of morphine dependent rats were established by intraperintoneal injection and combined with environmental stimulation. Rats received Quinpirole or Raclopride injection (agonist or antiagonist of the DA D2 receptor) in OFC by a minim-injector after the last conditioning session and CPP was assessed by the time spent in the morphine-associated environment after conditioning. record the time in drug place. All dates were analysed by statistical method . We research the effect of intervene by DA D2 receptor on morphine dependence.Result: The time in drug place of Raclopride intervene group exhibited respectively significantly longer than the control group after the 2nd withdrawal day(p < 0.05), however the time of Quinpirole intervene group didn't show significant changing than control group .Conclution: 1.CPP models of morphine dependent rats were established successfully by intraperintoneal injection with the dosage we explored by experience from referring many paper . 2.The experiment results indicate that the DA system in OFC plays an important role in the procession of morphine dependence. And this results have not been seen to report in the word at present. 3. The result indicate that the damnifing in orbital frontal cortex of animals who had addicted in drugs will increase their drugs craving behavior. In clinic, it can indicate that we must operate to intervene the patients of drug addiction cautiously .