| Microfluidic device (or called Lab-on-a-chip) is one of the important technicalplatforms in systems biology. In this thesis, we focus on the application ofmicrofluidic device in the field of gene analysis in order to develop the relatedtechniques of potentially biomedical significance. Six chapters are included.In the first chapter, the progress of microfluidic device and its development in thearea of gene analysis and apoptosis are described. During the second to fifth chapter,the practicability of in-house built microfluidic systems for gene analysis, dependingon the pathway of polymorphism analysis, foreign gene detection and geneexpression, are discussed. The work mainly include: 1) The polymorphism analysisof angiotensinogen gene in the core region of –6 A/G in 123 patients with essentialhypertension in dalian district; 2) The short tandem repeat polymorphism analysis ofginseng species and the further application of this technology in the identification oforiental ginseng and American ginseng. 3) The fast detection of PCR products fromtuberculosis, HCV virus, and human leukocyte related antigen (HLA-B27) gene; and4) the estimation of RNA from tissues or cells, and fucosyltransferase geneexpression from different mouse tissues.As apoptosis is of great important in the diagnosis and treatment of diseases, thesixth chapter focuses on the application of microfluidic chip in the detection of keyapoptotic events during arsenic trioxide-induced apoptosis of APL cells, includingthe analysis of redox states, DNA fragmentation et al. The simultaneousdetermination of reactive oxygen species and reduced glutathione in apoptotic cellsare realized, which indicates the potential functionality of microfluidic platform forapoptosis study.
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