A Study Of Lymphagiogenesis And Neck Lymph Node Metastasis In Squamous Cell Carcinoma Of Tongue

Uncontrolled recurrence and regional lymph node metastasis are the main lathel factors of squamous cell carcinoma of tongue. Cure rate and survival rate can not been improved over last two decade, though methods of clinical diagnosis and treatment have more development. At present, we can not get the more effective method of diagnosis and treatment, facing neck lymph node metastasis. The mechanism of lymphatic metastasis is indefinite. We have little known about the variation and contribution of lymphatic vessel of tongue cancer. Based on the retrospective analysis of squamous cell carcinoma of tongue , we observed the ultrastructure of intra-tumoral lymphatic vessel, investigate the role of lymphangiogenesis in tumor metastasis, and analysis the adjustment action of VEGF-C/VEGFR-3 in lymphangiogenesis one after another, lastly, we set up a tongue orthotopic transplantation of nude mouse. 1. Retrospective analysis of squamous cell carcinoma of tongue Objective Investigate relationship between clinical date and neck lymph node metastasis Methods Retrospective analysis data of 55 cases of tongue cancer by life table method and Kaplan-Meier method, establish Cox model to screen the prognosis factor Results 55 cases of tongue cancer were collected. Male to female is 1.39:1. Age of onset is from 31 to 82, the middle age is 59. The cases under 45 years old is 18.2%. Histopathologic lymph node metastasis were found in 47.27% (26/55) in total patients. Skip metastasis were found in 16.36% (9/55) in total patients. Overall survival rate of five years is 54.53%. Survival rate of patients who were found lymph node metastasis droped down to 24.2%, compared with patients without neck lymph node metastasis. Age of onset and sex is not relative with mortality. Tumor TNM, differenation is effective for evaluating lymphatic metastasis. But in Cox model, only neck lymph node metastasis is the independent prognostic factor. Conclusion Neck lymph node metastasis is the main prognostic factor of tongue cancer. 16.3% cases were found neck skip metastasis. Lympha node in Submaxillary and cervicales profundae superiorsregion are usually involved in. morbidity shows increasing frequency in youngpeople.2. Lymphangigenesis and neck lymph node metastasis in squamous cell carcinoma of tongue2.1 Lymphangiogenesis in squamous cell carcinoma of tongueObjective Investigate if lymphangiogenesis occur or not, and the relationship between lymphangiogenesis and neck lympha node metastasis. Methods Used LYVE-1 as the marker of lymphatic endothelial cell , compared to CD34,CD105, the markers of blood vessel in immunohistochemistry test., we collected the data of lymphatic microvessel density (LMVD)and blood vessel density (MVD)in cancer nest and cancer stroma under microscope, and measure the cancer stroma lymphatic vessel opening area by image analysis system of computer. Results There are no cross stain in lymphatic vessel and blood vessel marked by LYVE-1, CD34and CD 105. Statistical analysis Intra-tumoral LMVD 5.66±2.34 and normal tissue LMVD 3.18± 0.88, there is signification between them. In neck lymphatic metastasis group, indexes of LMVD and MVD in caner stroma and intra-cancer nest and the lymphatic vessel opening area are obviously higher than in non-metastasis group. Statistical analysis by logistic method, show LMVD and MVD in cancer stroma is signification between metastasis and non-metastasis group. Conclusion We found that LYVE-1 is the special marker of lymphatic endothelial cell. Lymphangiogenesis do occur in tongue cancer. LMVD and MVD in cancer stroma and intra-cancer nest, index of lymphatic opening area in cancer stroma, all relate with the status of neck lymphatic metastasis. Multiplicity shows LMVD in cancer stroma is the first risking index of neck lymphatic metastasis. 2.2 Observing ultrastructure of lymphatic microvessel in squamous cellcarcinoma of tongueObjective Observing ultrastructure of lymphatic microvessel in tongue cancer, investigate the relation between its construction features and neck lymphatic meta-stasis Methods 5 sample of SCCOT were investigate...