Quantitative Analysis Of Osteoblast And Periodontal Ligament Cell Behaviour On Two Kinds Of Titanium Substrata

Implant denture is used more and more. But something question is still puzzling people. For example, osseointegration of the osteoporosis, parenchyma interface of the implant, stress concentrating on the implant. All of these have respect to the geometric design and chemical compositions of an implant surface, it may have an important part in affecting early implant stabilization and influencing tissue healing.There is too much Interference factor in the experimentation inside. So experimentation in vitro is effective, it is not only a way to discover the connection of environment inside and cellular Variety, but also a method to improve the surface of implant. Through the experimentation in vitro we can find the osteoblast multiply and mineralize on the implant surface, and the periodontal ligament cells also, it is maybe a good way to conceive the periodontal ligament around the implant, and it maybe solve the problems of parenchyma interface of the implant, stress concentrating and so on.We evaluated the topographic effects of class hydroxyapatite(CHA) and titanium(Ti) surface that had identical mircropattems and to determine whether there was synergistic interaction between surface chemistry and topography.Osteoblast and periodontal ligament cells were seeded and cultured on the hydroxyapatite(CHA) surface and titanium(Ti) surface for up to 7~10 days, respectively. The cells' behavior was examined using: three- dimensional culture; immunofluorescence staining; Growth curve; MTT; ALP activity and Fluorescence activated cell sortor(FACS). Compare the effection of hydroxyapatite(CHA) surface and titanium(Ti) surface on cells.We found that rough surface was propitious to the attachment of cells, and it also offered the three-dimensional support to cell attachment, proliferation, and differenitiation. It is benefit to the cells' mineralization on the surface. Not only osteoblast, but also periodontal ligament cells, had the ability of mineralization on the hydroxyapatite(CHA) surface and titanium(Ti) surface. Especially the former had the better ability of osteoinduction.