| Sixty rats were treated with cyclosporine A (CsA , 30mg/kg.d), taclimus (Tac, 0.6mg/kg.d) and saline (3ml/kg.d)respectively. Serum and splenocyte samples were used to evaluate the cytokine (IL-18 and IL-10) response. Paraffin-embedded kidney transplant nephrectomy specimens were conducted to examine IL-18 receptor, CD30 and Fas ligand (FasL) expression by ultra-sensitive S-P immunohistochemical staining .The expression of CD4/IL-18Rα, CD8/IL-18Rα and CD3/FasL on peripheral lymphocytes were examined using flow cytometric analysis in renal transplantation recipients before and after renal transplantation. The results showed that inhibited serum and splenocyte IL-10 may be one of the causes of tacrolimus with more powerful immunosuppressive. The local expressions of IL-18R, CD30 antigen and FasL were stronger significantly in renal allograft lessions induced by AR and HAR. Detection of CD4/IL-18Rα, CD8/IL-18Rα and CD3/FasL on the peripheral lymphocytes by flow cytometry was an easy, rapid, and noninvasive means for the diagnosis and treatment of patients with acute rejection after renal transplantation.
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