Effect Of La Protein And Its Mutants On The Transcription Translation Initiation And Replication Of Hepatitis B Virus

The aim of this study was to investigate the effect of La and mutants on binding affinity, transcription, translation initiation of HBV RNA and HBV replication. Wild La, mutants and eukaryotic expression plasmid of adr HBV, ORF-P, ORF-S, ORF-C and ORF-X were constructed to research the influence of La or mutants on mobility of HBV RNA, HBV ORF transcription and HBsAg, HBeAg expression by EMSA and in vitro transcription-translation system. The relation of La and HBV replication was studied in HepG2.2.15 cell by RNAi. It suggested: the affinity of wild La and HBV RNA was perfect and different mutant sites influenced the binding affinity;the deletion of RRM3 had significant effect;wild La up-regulated HBV, ORF-P, ORF-C transcription and HBsAg expression, but it inhibited ORF-X transcription and HBeAg expression;DeL2 inhibited ORF-X transcription, but DeL2 and DeL3 promoted HBeAg expression notably;it was positive correlation between HBsAg, HBeAg, HBV DNA and La in cells. These results indicated: RRM3 was connected with the binding function;RRM3 and RRM2 were involved in transcription and translation initation regulation. It will be a novel antiviral strategies by studying the interaction of La and HBV.