The Study About The Proliferation Effect Of Kudzuvine Root Extractive To Endometrial Cancer Cell In Vitro

Objective: endometrial cancer(EC),carcinoma of corpusuteri,is one of the malignant tumor in the most frequent female genital tract disease. It occurs easily in 58-61 years old. It is about 7% in the female tumor and 20-30% in the tumor of the female reproductive system. EC is one of the estrogen dependent malignant tumor causing by endometia. Recently, the disease incidence is increasing year by year, with hormone replacement therapy (HRT) generally being used. It get close, even exceed, to cancer of the cervix. Although there are many research institute going in for rsch of EC, The pathogenesy is inadequacy identified. It's necessary to study more deeply. For this reason, this study's object is endometrial cancer cell line Ishikawa. We want to identify the mechanisms of EC, investigate the mechanisms of inhibiting proliferous effect of endometrial cancer cell line Ishikawa treated with Genistein in vitro and offer rationale and experimental data for treating EC by TCM.METHODS:1,To detect the effect of inhibiting proliferous to Ishikawa cells treated with Genistein by MTT respectively: After different density Genistein treating to Ishikawa cells 48 hours, we evaluated photoabsorption value, calculating inhibition ratio and getting quantity and effect data. After treating Ishikawa cells by IC₅₀ BGenistein 24 hours,48hours,72hours,96hours,120hours, we evaluated photoabsorption value, calculating inhibition ratio and getting seasoning data.2,To detect the effect of cell cycle and apoptosis to Ishikawa cells treated with Genistein by flow cytometry. After different density Genistein treating Ishikawa cells 48 hours, fixing cells, digesting half an hour by RNAase, dyeing 1 hour by PI away from light, we detected it, analyzeed cell cycle by ModiFit software and draw a DNA profile.3,To detect the effect of Bregulating to ERαBmRNA of Ishikawa cells treated with Genistein, E₂ and P₄ by RT-PCR method: After different density Genistein, E₂ and P₄ treating Ishikawa cells 48 hours, we extracted RNA, did RT-PCR reaction, collected image by electrophoresis and gel image analytical apparatus and analyzed gray scale.Result:1,Genistein markedly inhibited proliferation of Ishikawa cell in vitro. Its...