Effect Of Expression Of P53 Gene Driven By HTERT Gene Promoter On T24 Apoptosis

Therapeutic gene expression in tumor cells driven by tissue specific promoter can decrease damage to normal tissue. Normal p53 expression suppresses cellular proliferation, promotes apoptosis. Human telomerase reverse transcriptase (hTERT) has the character that it expresses only in tumor cells that telomerase were active, which makes it an important tool for targeting gene therapy. Wild-type p53 gene can inhibit cell proliferation and induce apoptosis. p53 mutation in tumor cells is considered one of the pathogen of tumor progression. We constructed the expression vector of wide-type p53 gene afforded by hTERT promoter, transiently transfected into T24 cells and investigated the effect on cell cycle and proliferation of tumor cells by using FCM and MTT method. Expression of p53 driven by hTERT promoter increased apoptosis rate (apoptosis rate after 24 /48 hours: p53: 15.42%/36.57%;EGFP: 5.17%/8.19%;negative control: 4.49% /7.18%) and inhibited proliferation (proliferation inhibited rate: p53: 47.5%;EGFP: 2.8%;negative control: 2.5%). The expression of p53 gene under the control of hTERT promoter can increase apoptosis and inhibitory rate of bladder carcinoma cells, and this strategy can be considered an option of gene targeting therapy with high specificity.