Study On Construction Of Retrovirus Vector Taking MDR1 Gene And Its Transfection Into Mesenchymal Stem Cells Derived From Human Placenta

The chemotherapy occupies an extremely important position in the treatment of gynecology malignant tumor.Some gynecology familiar malignant tumors,such as malignant trophoblastic tumor,ovary cancer and endometrial cancer, are impressionable to chemotherapy,among them, malignant trophoblastic tumor is the disease that can be cured merely by chemotherapy.However, repeatedly and large dose chems will cause the occurrence of marrow represses and drug fast.An utility pathway to protect hematopoietic cells under chems is very important to increase the therapeutic efficacy of malignant tumors.Thansfection of multidrug resistance gene into haemopoietic stem cells in bone marrow can protect haemopoietic stem cells and peripheral blood leucocytes from large dose chems,while tumor cells die of the chems.But the low transfection efficiency of haemopoietic stem cells limits the development of this techniqe.MSCs coexist with haematogenesis stem/ progenitor cells in marrow. marrow derived MSCs have been shown expressed hematopoietic cytokines to support hematopoietic stem/progenitor cells (HSPC) expression ex vivo...