Study On The Mechanism Of The Regulation Of Pancreatic External Secretion By Nerve Transfer

Pancreas is both an exocrine and endocrine organ which can secrete pancreatic juice which is very important for the digestion of food. Now, most of the pancreatic diseases have the result of change of pancreatic exocrine secretion. So it is thought to be very important in the control of pancreatic exocrine secretion. Recently, nerve control was thought to be very important in the control of pancreatic exocrine secretion, especially vagus and dorsal motor nucleus. And this study aims on the learning of the mechanism of the neurotic transmit on the control of pancreatic secretion.1. The duodenal stimulation of 5-HT induced c-Fos, Glu-R, SP-R expression in the ganglions of vagus in ratsObjectives: This part was to investigate the activity of visceral sensory neurons in the ganglions of vagus after the duodenal stimulation of some different kinds of stimulation. Methods: Rats were divided into 4 groups: Sham operation, deionized water,5%NaCl,10^-4mol/L5-HT,and10 rats in every group. Then Immunohistochemical staining ,quantitative analysis of c-Fos were achieved in all kinds of groups, and so did the NK1-R, NMDA-R2ab in the c-Fos positive neurons. Results:the duodenal stimulation by 5%NaCl,10^-4mol/L5-HT induced significant c-Fos expression with the NK1-R,NMDA-R2ab expressions. Conclusions: during the duodenal stimulation, 5%NaCl,10^-4mol/L5-HT helped transfer the signal to the ganglions of vagus and express Glu-R and SP-R there.2. The converse trace of the DMNObjectives: This part was to investigate the activity of visceral sensory neurons in DMN after the duodenal stimulation of 10^-4mol/L çš„ 5-HT stimulation and the injection of CTB into the pancreas. Methods: Rats were divided into 3 groups: A: 10 rats, with CTB injection and stimulation;B: 1 rat, with CTB injection and nostimulation;C:l rat, with Saline injection and stimulation. Then the Immunohistochemical staining , quantitative analysis of CTB were achieved in all kinds of groups, and so did the c-Fos, ChAT, NK1-R, NMDA-R2ab in the CTB positive neurons. Results: A: CTB+, c-Fos, ChAT> NK1-R ft NMDA-R2ab were all well expressed;B: CTB-, others were also negative;C: CTB-, others were positive. Conclusions: during the duodenal stimulation, lO^mol/LS-HT helped transfer the signal to the DMN and express c-Fos, CIiATn NK1-R, NMDA-R2ab well there.3. The effect of microinjection of SP,NPY,SS into the DMN on the control ofthe pancreatic exocrine secretionObjectives: To investigate the effect of microinjection of SP,NPY,SS into the DMN on the control of the pancreatic exocrine secretion. Methods: Rats were divided into 4 groups: A: 10 rats, with aCSF injection;B: 10 rats, with SP injection;C: 10 rats, with NPY injection;D: 10 rats, with SS injection. Then the Immunohistochemical staining , quantitative analysis of c-Fos in the pancreatic neurons were achieved in all kinds of groups, and so did the ChAT,GRP in the neurons. Results: SP and NPY stimulated the pancreatic neurons to express c-Fos, with ChAT and GRP, But SS suppressed the pancreatic neurons to express c-Fos, with little ChAT GRP expression. Conclusions: SP and NPY effect on the DMN to promote the pancreatic exocrine secretion, but SS effects on the DMN to restrain the pancreatic exocrine secretion.