The Studies On Molecular Biological Character And Operative Therapy Of Pituitary Adenomas

Objective Study the expression of nucleostemin, ASPP2 gene mRNA and Ki-67 antigen in pituitary adenomas, analyze the relationship between the expression and clinical data, such as invasion, tumor diameter, pathology type, and so on. Assay the function of nucleostemin and ASPP2 gene on cell proliferation, tumorigenesis and developing in pituitary adenoma further. Methods 71 samples of pituitary adenomas were collected. Semi-quantitative reverse transcriptase-polymerase chain (RT-PCR) was used to detect expression of nucleostemin, ASPP2 gene mRNA in samples. Immunochemistry Technique was applied to examine Ki-67 expression in the paraffin section of samples. At the same time, coherent clinical data were collected. Results (1) Nucleostemin gene mRNA were detectable in all samples of pituitary adenomas. Nucleostemin expression was the highest in PRL adenoma, and it's the lowest in nonfunctional adenoma, but there was no statistical significance among different pathological type (P>0.05). (2) ASPP2 gene mRNA were detectable in all samples of pituitary adenomas. ASPP2 expression was the lowest in PRL adenoma, and it's the highest in GH adenoma, but there was no statistical significance among different pathological type (P>0.05). (3) Ki-67 LI was the highest in PRL adenoma, the lowest in nonfunctional adenoma, when contrast with each other, its of great statistical significance (P<0.01). Difference between PRL adenoma and GH adenoma was of statistical significance(P<0.05). Difference of Ki-67LI in nonfunctional adenoma, GH adenoma and multi-hormone adenoma was of statistical significance(P<0.05). (4) The patients' age among different pathological types of adenoma is different(P<0.05). Average age of gonadotroph adenoma patients was the largest, ACTH adenoma patients' was the lowest. When contrast with each other, there existed great statistical significance (P<0.01). Patients average age of gonadotroph adenoma and GH adenoma was also different (P<0.05). (5) Nucleostemin, ASPP2 mRNA and Ki-67LI expression difference between apoplexia and non-apoplexia pituitary adenoma were not significant (P>0.05). (6) Patients' age,tumor average diameter, nucleostemin mRNA, ASPP2 mRNA and Ki-67 expression were higher in invasive pituitary adenoma than that in noninvasive pituitary adenoma. Differences of tumor average diameter, nucleostemin and ASPP2 mRNA expression between them were extremely significant(P<0.01). And that of age and Ki-67 LI were significant. When compared with course and sex, they had no significant difference(P>0.05). Invasion was no significant difference between functioning and non-functioning pituitary adenomas(P>0.05). (7) There was 5 cases recur in the 64 follow-up patients (7.8%). Tumor average diameter, course, nucleostemin gene expression and Ki-67 LI were higher in recurrent group than that in non-recurrent group. Statistical results showed that difference of Ki-67 LI between them was significant(P<0.05), but other factors were of no statistical significance(P>0.05). (8) There was positive correlation between nucleostemin gene and KJ-67LI level(P<0.05). Correlation between ASPP2 and Ki-67LI was negative(P<0.05). A negative correlation was demonstrated between nucleostemin and ASPP2 expression(P<0.01). (9) Age and mean tumor diameter were significantly higher in men than in women (PO.001). Conclusions (1) Nucleostemin and ASPP2 gene might express in human pituitary adenoma tissues. (2) the expression differences of nucleostemin ASPP2 and Ki-67 in different pathological types of pituitary adenomas, represented differences in molecular biological character, relating with invasion. (3) In different pathological types of pituitary adenomas, the age existed difference, which shows that different age might have predilection in pathological type. (4) In pituitary adenoma, nucleostemin expression had positive correlation to tumor proliferation, thus ASPP2mRNA expression had negative correlation to tumor proliferation. (5) With higher nucleostemin and Ki-67, lower ASPP2, elder age, larger maximum diameter, the tumor had higher probability ratio to invasive. Tumor probability ratio to invasive had no difference in patients of different sex. (6) Ki-67LI might be a valid clinical detection marker to decision pituitary adenoma relapsing. (7) Among raising up of nucleostemin gene expression, depressing of ASPP2 gene expression and high Ki-67, there existed associativity in pituitary adenoma. (8) in patients of different sex, the age of morbility was difference.