Effects Of Rehmannia Glutinosa Oligosaccharides On Autologous Skeletal Myoblasts Transplantation In Swine With Myocardial Infarction

Background:There are some salient relations between the theory of congenital spirit (Xian Tian Zhi Jing) in Chinese tradition medicine (CTM) and the theory of stem cells in modern medicine. The category of kidney(shen) in CTM is somewhere in which stores the "Xian Tian Zhi Jing". CTM materials (such as Rehmannia Glutinosa) with function of tonifying the "Shen", replenishing "Qi" and enriching the"Xue" would effect to the physiological characteristic of stem cells.Objective:To evaluate the effects of Rehmannia Glutinosa Oligosaccharides (RGOS) on autologous skeletal myoblasts (SMs) transplantation in swine with myocardial infarction and to discuss the pharmacological actions of RGOS to SMs in vitro. Methods and results:Crude extract RGOS was extracted by boiled water from Rehmannia Glutinosa and was further separated by charcoal column chromatography. The stachyose, the main component in the GROS, was determined by High Performance Liquid Chromatography (HPLC). The output of crude extract RGOS from raw material is 22.17%, and purified RGOS contained 65.15% stachyose. In vitro, SMs were procured by a modified method from adult SD rats. RGOS could accelerate proliferate of SMs and advance its IGF-II secretion. Furthermore RGOS could elevate Ito current of SMs and protect the SMs from apoptosis aroused by hypoxia. However, RGOS could not promote the SMs express specific protein of myocardium cell. RGOS in 0.625mg/ml had the best cost-effectiveness. In Vivo, SMs were procured from swine, nine Swines were divided into 3 groups, group A (MI), group B (SMs), Group C (SMs combination RGOS);. Myocardium infarction (MI) model was obtained through left anteriordescending branch obstruction and SMs (1x10 cells) were injected locally into MI region after 14 days of MI in groups B and C. The group C were fed with RGOS 4g/d. After 30 days cellular transplantation, all the animals were examined in left ventricular ejection fraction (LVEF), tissue doppler imaging (Sm,Sa), IGF-II, TNI, CK-MB and CD34+ cells in blood before killed to get histological study. The results showed newborn striated muscle tissue was more inclined to gather in MI region in the local injection. SMs transplantation could mend the LVEF and Sa which were impaired by MI, and RGOS could augment the treatment efficiency through increasing IGF-II, CD34+ cells in blood, promoting marrow hyperplasia and anti-apoptosis in SMs preparation;meanwhile, RGOS could decrease the arrhythmia 24 hours after cellular transplantation. Conclusions:As a kind of SMs accessoria peri-transplantation 1 therapy, RGOS could provide a novel strategy for cellular cardiomyoplasty for cardiac repairment.