1.Cloning And Analysis Of Energy Metabolism-related Gene HSDCT2 From Human Kidney By The Aid Of Bioinformatics 2.The Changes Of Gene Expression Profile In The Aging Kidney And After The Treatment Of SLE With Traditional Chinese Medicine By Gene Chip

To study the physiological function and the relationship of energy metabolism-related human high-affinity sodium-dependent dicarboxylate co-transporter protein (hSDCT2) with the pathogenesis of human diseases, hSDCT2 gene was successfully cloned from human kidney by the aid of bioinformatics. Firstly, rat SDCT2 cDNA was compared with human EST database to obtain EST fragments with high homology;Then these ESTs were assembled into one 2540 bp contig by DNAstar software. Based on the contig sequence, PCR primers were designed and hSDCT2 cDNA was amplified from human kidney total RNA by RT-PCR and sequenced. Sequencing results showed that hSDCT2 cDNA contained 1809 bp full-length open reading frame to encode 602 amino acids. Homology analysis indicated that amino acid sequence of hSDCT2 protein had 85% and 87% consensuswith rat and mouse SDCT2, respectively. The prediction of second structure for hSDCT2 protein showed that the protein contained 12 putative a-helix transmembrane domains. SAGEWirtual Northern analysis suggested that the gene was expressed in human kidney> livers placentas brain> lungs colon and central nervous system, etc. hSDCT2 gene was located on chromosome 20ql2-13.1 by HTGS and STS/GDB. Therefore, the on-line gene cloning based on informatics is rapid and accurate cloning approach without wasting to much time and money.